NDRG 1基因与宫颈鳞癌发生发展及转移的关系  

The relationship between NDRG-1 gene expression and the progression and metastasis of uterine cervical squamous cell carcinoma

作　　者：马少飞[1] 陈嘉薇[1] 祝峰[1] 

机构地区：[1]上海交通大学附属上海市第一人民医院病理科,上海200080

出　　处：《肿瘤》2008年第3期238-241,共4页Tumor

基　　金：上海市科学技术委员会基金资助项目(编号:04JC14064)

摘　　要：目的:探讨N-myc下游调节基因(N-myc downstream reglated gene1,NDRG1)与宫颈鳞癌发生发展及转移的关系。方法:采用免疫组织化学ABC法检测42例宫颈鳞癌组织、30例宫颈高级别上皮内瘤变组织、28例宫颈低级别上皮内瘤变组织及20例正常宫颈上皮组织中NDRG1蛋白的表达。RT-PCR检测10例新鲜宫颈鳞癌组织与10例正常宫颈上皮组织中NDRG1mRNA的表达。结果:宫颈鳞癌组织中NDRG1mRNA和蛋白的表达较正常宫颈上皮组织明显降低(P<0.01)。从正常宫颈上皮到低级别上皮内瘤变、高级别上皮内瘤变、再到宫颈鳞癌的发生发展过程中NDRG1蛋白的表达水平逐渐降低,差异有统计学意义(P<0.01)。有转移的宫颈鳞癌组织中NDRG1mRNA与蛋白的表达较无转移组明显降低(P<0.05)。结论:NDRG1mRNA与蛋白在宫颈鳞癌组织中的下调表达提示NDRG1基因可能与宫颈鳞癌的发生发展及转移有关。Objective ：To investigate the relationship between N-myc downstream-regulated gene-1 （NDRG-1） gene expression and the progression and metastasis of uterine cervical squamous cell carcinoma （UCSCC）. nethods：NDRG-1 protein expression was detected by immunohistochemistry （IHC） in 42 cases of UCSCC, 30 cases of high-grade squamous intraepithelial lesion （HSIL）, 28 cases of low-grade squamous intraepithelial lesion （ LSIL）, and 20 cases of normal squamous epithelium. NDRG-1 mRNA was detected by reverse transcriptase-polymerase chain reaction （RT-PCR） in 10 cases of UCSCC and 10 cases of normal squamous epithelium. Resuits：The expressions of NDRG-1 mRNA and protein in UCSCC were significantly lower than normal squamous epithelium tissues （P 〈 0.01 ）. From normal squamous epithelium to LSIL, HSIL and UCSCC, NDRGI protein was gradually decreased during tumor carcinogenesis. The difference was significant （P 〈 0.01 ）. NDRG-1 mRNA and protein in metastatic UCSCC were obviously lower than non metastatic UCSCC （P 〈 0.05 ）. Conclusion： Down-regulation of NDRGI mRNA and protein in UCSCC indicate that NDRGI gene might correlate with carcinogenesis, progression, and metastasis of cervical squamous carcinoma.

关 键 词：宫颈肿瘤 基因表达 免疫组织化学 反转录酶链反应 

分 类 号：R737.33[医药卫生—肿瘤] R730.231[医药卫生—临床医学]