XRCC 1 Arg399Gln基因多态性对铂类药物化疗胃癌患者预后的影响  

The prognostic impact of XRCC1 Arg399Gln polymorphism on prognosis of gastric cancer patients treated with platinum-based chemotherapy

作　　者：黄朝晖[1,2] 华东[1] 李莉华[1] 吴小红[1] 宋明旭[1] 刘志辉[1] 周希科[1] 朱景德[2] 

机构地区：[1]苏州大学附属第四医院肿瘤研究所,无锡214062 [2]上海市肿瘤研究所,癌基因及相关基因国家重点实验室,上海200032

出　　处：《肿瘤》2008年第3期242-245,共4页Tumor

基　　金：无锡市社会发展科技指导性计划项目(CLZ00612)

摘　　要：目的:研究DNA修复酶X射线损伤交叉互补蛋白1(X-ray repair cross-complementary protein1,XRCC1)基因Arg399Gln多态性对接受铂类药物辅助化疗的胃癌患者预后的影响。方法:选取经病理学确诊的胃癌患者84例,采用含铂类药物的联合化疗方案进行辅助化疗。化疗前采集患者外周血,采用聚合酶链反应-连接酶检测反应(polymerase chain reaction-ligation detection reaction,PCR-LDR)技术检测XRCC1基因Arg399Gln的多态性。结果:84例胃癌患者中,49例(58.3%)携带XRCC1的399Arg/Arg基因型,30例(35.7%)携带Arg/Gln基因型,5例(6.0%)携带Gln/Gln基因型;截止到随访结束,分别有63.1%(53/84)和55.2%(43/84)的患者出现复发和死亡,其中携带Arg/Arg基因型的胃癌患者复发率和死亡率均显著低于携带Arg/Gln或Gln/Gln基因型患者(P<0.05)。COX多因素分析显示,携带Arg/Arg基因型的患者具有较好的无复发生存期和总生存期预后(P<0.05)。结论:XRCC1Arg399Gln基因多态性与术后胃癌患者接受铂类药物化疗后的预后有关,可以在一定程度上判断术后胃癌患者接受铂类药物化疗的预后情况。Objective ： To investigate the association of DNA X-ray repair cross complementary protein 1 （ XRCC1 ） Arg399Gln polymorphism with the clinical outcome of gastric cancer patients treated with platinum-based chemotherapy. Methods： Eight-four patients with gastric cancer confirmed by pathological examination received postoperative combined chemotherapy （ including platinum）. Transcription of XRCC1 Arg399Gln was determined by polymerase chain reaction-ligation detection reaction （PCR-LDR）. Results： Of the 84 patients, the frequencies ofXRCC1 codon 399 Arg,/Arg, Arg,/Gln, and Gin/Gin genotype were 58.3% （49/84）, 35.7% （30/ 84） , and 6.0% （5/84）, respectively; and the relapse and death rate were 63.1% （53/84） and 55.2% （43/84）, respectively. The relapse and death rates in patients with Arg/Arg genotype were significantly lower than those in patients with Arg/Gln or Gln/Gln geno- types （P 〈 0.05 ）. COX multivariate analysis showed that XRCC1 Arg399Arg polymorphism was associated with better recurrence-free and overall survival in gastric cancer patients compared with other genotypes （ P 〈 0.05 ）. Conclusion： Polymorphism of XRCC1 Arg399Gln correlated with the prognosis of gastric cancer patients treated with postoperative platinum-based chemotherapy. It may be helpful to a certain extent for evaluating the prognosis.

关 键 词：胃肿瘤 化学疗法 多态性 单核苷酸 DNA修复酶类 X射线损伤交叉互补蛋白1 

分 类 号：R735.2[医药卫生—肿瘤]