低咖啡因茶树品系的RAPD分析及特异片段的克隆  

Analysis of Low Caffeine Tea Lines by RAPD and Cloning of Special Molecular Markers

作　　者：王会[1] 梁月荣[1] 陆建良[1] 

机构地区：[1]浙江大学茶学系

出　　处：《茶叶》2008年第1期29-32,36,共5页Journal of Tea

基　　金：国家自然科学基金(30771374)资助项目

摘　　要：对12份来源不同和咖啡碱含量不同的茶树种质资源进行RAPD分析,筛选出品种(株系)唯一扩增的RAPD位点16个,这些位点可以作为品种(株系)鉴定的特异分子标记;对S43.04序列进行测序,该序列为752bp的核苷酸序列,具有编码40个氨基酸的开放阅读框,其相同氨基酸残基与β-半乳糖苷酶的同源性为72%。Twelve lines which were introduced from various estates and had various concentrations of caffeine, were investigated by RAPD technology, in which 16 molecular markers specifically amplified on single lines were identified and the molecular markers could be used as indicators identifying related tea cultivars or lines. Some of the specific loci were sequenced, for example the band S43.04 had 752 bps with a reading frame encoding 40 amino acids. The amino acids sequence had 72% identity with the gene encoding β-galactosidase. Key words

关 键 词：株系 特异分子标记 测序 RAPD 

分 类 号：S571.1[农业科学—茶叶生产加工]