皮肤癌组织中环氧合酶-2和缺氧诱导因子-1α的表达及微血管密度测定  

Detection of COX-2,HIF-1α and microvessel density in cutaneous carcinoma tissue

作　　者：赵丽[1] 刘林嶓[2] 

机构地区：[1]郑州市第一人民医院整形外科,郑州450004 [2]郑州大学第一附属医院整形外科,郑州450052

出　　处：《郑州大学学报（医学版）》2008年第2期210-212,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关基金资助项目0124170636

摘　　要：目的:检测皮肤鳞状细胞癌(SCC)和基底细胞癌(BCC)组织中环氧合酶-2(COX-2)和缺氧诱导因子-1α(HIF-1α)的表达,分析皮肤癌组织中2者的相关性及与血管生成的关系。方法:采用免疫组织化学方法检测25例SCC、25例BCC及23例正常皮肤组织中COX-2、HIF-1α和CD34的表达,并计数CD34标记的微血管密度(MVD)。结果:SCC和BCC组织中COX-2和HIF-1α蛋白阳性表达率及CD34-MVD均高于正常皮肤组织(P<0.05)。COX-2和HIF-1α的表达相关(P<0.05)。SCC和BCC组织中COX-2、HIF-1α表达阳性者的MVD值均高于表达阴性者(P<0.05)。结论:SCC和BCC组织中COX-2与HIF-1α可能协同促进肿瘤新生血管生成,从而促进SCC和BCC的生长、浸润或转移。Aim ： To investigate the expression of cyclooxygenase-2 （ COX-2）, hypoxia-inducible factor-1α （HIF-1α） and the correlation with angiogenesis in cutaneous squamous cell carcinoma （SCC） and basal cell carcinoma （BCC） tissue. Methods：The SP immunohistochemistry method was used to detect the expression of COX-2, HIF-1α and CD34-MVD in 25 cases of SCC, 25 cases of BCC, and 23 cases of normal skin tissue. Results： The positive expression rates of COX- 2 and HIF-1α,and MVD marked by CD34 in SCC and BCC tissue were significantly higher than those in normal skin tissue （P 〈0.05）. The expression of HIF-1α was related to the expression of COX-2 in SCC and BCC tissue（P 〈0.05）. The cases with positive expression of COX-2 and HIF-1α had higher MVD （P 〈 0.05）. Conclusion ：There are overexpression of COX-2 and HIF-1α in BCC and SCC. They probably have a synergistic effect in the neovascularization of the tumor, thus promote the carcinogenesis and development of SCC and BCC.

关 键 词：皮肤肿瘤 鳞状细胞癌 基底细胞癌 环氧合酶-2 缺氧诱导因子-1Α 微血管密度 

分 类 号：R739.5[医药卫生—肿瘤]