Ⅰ型神经纤维瘤组织中神经纤维素、Ras-p21蛋白的表达  

Expression of neurofibromin and Ras-p21 protein in neurofibromas tissue from neurofibromatosis type 1 patients

作　　者：王伟[1] 翟晓梅[1] 

机构地区：[1]郑州大学第一附属医院整形外科,郑州450052

出　　处：《郑州大学学报（医学版）》2008年第2期338-339,共2页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨Ⅰ型神经纤维瘤病(NF1)、孤立性神经纤维瘤患者瘤组织中神经纤维素、Ras-p21表达情况。方法:应用Western blot检测19例NF1、16例孤立性神经纤维瘤瘤组织及11例正常神经组织中神经纤维素、Ras-p21的表达。结果:NF1瘤组织中神经纤维素的表达水平低于孤立性神经纤维瘤瘤组织及正常神经组织,Ras-p21的表达水平高于孤立性神经纤维瘤瘤组织及正常神经组织(P均<0.05)。2者在孤立性神经纤维瘤瘤组织中的表达与正常神经组织比较,差异无统计学意义(P>0.05)。结论:NF1的发病可能与NF1基因突变引起神经纤维素缺失、导致其对Ras-p21的抑制作用减弱或缺失有关。病变组织中神经纤维素的检测可能有助于NF1的诊断和与孤立性神经纤维瘤的鉴别诊断。Aim ： To investigate the differences in the expression of neurofibromin and Ras-p21 in neurofibromas tissue of neurofibromatosis typel （ NF1 ） and solitary neurofibroma（SNF）. Methods：The expression of neurofibromin and Ras-p21 protein in neurofibromas tissue from 19 cases of NF1 and 16 cases of SNF were detected using Western-blot. 11 cases of normal nerve tissue were the control. Results： The expression of neurofibromin in neurofibromas tissue from NF1 group was significant lower than those from SNF group and the control group （ P 〈 0. 05 ）, while the expression of Ras-p21 protein was higher than those from SNF group and the control group （P 〈 0. 05）. No difference in the expressions of neurofibromin and Ras-p21 between the SNF group and the control group were found. Conclusion ： NF1 gene leading to the deletion of neuroflbromin which resulted in the deletion of Ras-p21 may be involved into the NF1 pathogenesis. The detection of neurofibromin may be helpful for NF1 diagnosis and differentiation to SNF.

关 键 词：Ⅰ型神经纤维瘤病 孤立性神经纤维瘤 神经纤维素 RAS-P21 

分 类 号：R739.4[医药卫生—肿瘤]