细菌脂多糖对体外培养大鼠中性粒细胞P38和JNK蛋白激酶表达的影响  

Effects of LPS on expressions of P38MAPK and JNKMAPK in cultured rat neutrophils in vitro

作　　者：徐涛[1] 曾邦雄[2] 李世忠[1] 

机构地区：[1]北京积水潭医院麻醉科,北京100035 [2]华中科技大学同济医学院协和医院麻醉科,武汉430022

出　　处：《郑州大学学报（医学版）》2008年第2期348-350,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:观察体外培养大鼠中性粒细胞经细菌脂多糖(LPS)刺激后P38蛋白激酶和JNK蛋白激酶的变化,并探讨其调节作用。方法:抽取SD大鼠外周血分离培养中性粒细胞,随机分为5组:对照组、30min组、60min组、90min组、120min组,除对照组外各组分别加入1mg/LLPS,刺激后制成细胞涂片,以免疫细胞化学法检测P38蛋白激酶和JNK蛋白激酶的表达。结果:LPS刺激后,P38蛋白激酶和JNK蛋白激酶表达均增加(P<0.05或0.01)。P38蛋白激酶60min组达到高峰,JNK蛋白激酶30min组达到高峰,与其他时点相比差异均有统计学意义(P均<0.05)。结论:LPS刺激体外培养的中性粒细胞可以引起P38、JNK蛋白激酶的变化,且JNK蛋白激酶变化更加迅速。Aim ： To observe the expression of P38MAPK and JNKMAPK in cultured neutrophils induced by LPS, and study the regulative mechanism. Methods： Neutrophils isolated and cultured from SD rats were divided into five groups： control group, 30 min group, 60 min group, 90 min group,and 120 min group. LPS（ 1 mg/L） was taken into every experimental group. By immunohistochemsity P38MAPK and JNKMAPK were measured. Results： LPS induced the positive neutrophils of P38MAPK increasd with a time-dependent way（ P 〈 0, 05 ）. The peak of P38MAPK reached in 60 min group, and the peak of JNKMAPK reached in 30 min group. There were significant difference compared with control group （P 〈 0. 05 or 0. 01 ）. Conclusion： LPS could induce changes of P38MAPK and JNKMAPK expression in neutrophil, and P38MAPK change is faster than JNKMAPK.

关 键 词：细菌脂多糖 中性粒细胞 P38蛋白激酶 JNK蛋白激酶 大鼠 

分 类 号：R364.5[医药卫生—病理学]