CD4^+T细胞对自体非霍奇金淋巴瘤细胞的体外诱导分化作用  

Effect of CD4^+ T cells on the induction of differentiation of autologous non-Hodgkin B-cell lymphoma cells in vitro

作　　者：王瑾[1] 糜坚青[1] 马立恒[1] 陈钰[1] 沈志祥[1] 陈赛娟[1] Pascal PERRON Jean-Jacques SOTTO Thierry BONNEFOIX 王振义[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院血液科上海血液学研究所,上海200025 [2]Département de Cancérologie et d’Hématologie,Centre Hospitalier Universitaire Michallon

出　　处：《内科理论与实践》2008年第2期126-130,共5页Journal of Internal Medicine Concepts & Practice

摘　　要：目的:研究浸润于肿瘤组织中的CD4+T淋巴细胞与自身B细胞非霍奇金淋巴瘤(B-NHL)肿瘤细胞间的相互作用。方法:采用磁珠抗体分离法将1例B-NHL患者的脾边缘区恶性B淋巴细胞与浸润在肿瘤组织中的CD4+T细胞分离出来,共培养并观察恶性B淋巴细胞的生物学变化。结果:恶性的B细胞发生了形态学变化,逐渐向浆细胞转化;同时,逐渐丧失其细胞膜表面特异性标记CD19等B细胞特征性的标记,取而代之的是CD138等成熟浆细胞特有的标记分子。通过抗体分离得到CD138+细胞后,经重组免疫球蛋白(Ig)重链的PCR进一步证实这些细胞来源于恶性B细胞。结论:低分化恶性B细胞的正常分化过程并非完全阻断,适当的外界微环境能启动受阻的分化过程。肿瘤组织来源的CD4+T细胞具有促使自体肿瘤B细胞向浆细胞分化的能力。Objective To investigate the interaction between CD4^＋ tumor infiltrating T lymphocytes （TIL-T） and autologous tumor cells of B-non Hodgkin＇s lymphoma （NHL） in co-culture system. Methods CD4^＋ TIL-T lymphocytes from TIL-T and malignant B cells from splenic marginal zone were isolated from one patient with B-NHL by magnetic beads coated with antibodies. Then after co-culture, the biological changes of malignant B cells were analyzed morphologically, immunologically and molecularly. Results Morphologicaly, the malignant B cells gradually differentiated into mature plasma cells. At the same time, these malignant B cells gradually lost their specific B cell surface marker CD19 and replaced by the specific marker of mature plasma cell CD138. The CD138^＋ cells obtained via isolation by immunologic method were proved to be originated from the malignant B cell by PCR of rearranged IgG heavy chain. Conclusions The normal differentiation process of low differentiated malignant B cells is not completely blocked, appropriate external microenvironment can promote the initiation of the blocked differentiation process. The CD4^＋ cells originated from tumor tissue have the ability to promote the differentiation of autologous malignant B cells into plasma cells.

关 键 词：CD4 T细胞 诱导分化 非霍奇金淋巴瘤 免疫治疗 

分 类 号：R733.1[医药卫生—肿瘤]