复合法诱发小鼠结肠炎模型的建立和免疫学验证  

Reproduction and immunological authentication of marine model of colitis induced by a combination of dinitrochlorobenzene and acetic acid

作　　者：安晓霞[1] 崔玉芳[1] 李燕[1] 董波[1] 孙淑华[1] 莘旭妮[1] 柳晓兰[1] 毛建平[1] 刘萍[2] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]解放军总医院,北京100853

出　　处：《感染．炎症．修复》2008年第1期28-31,F0003,共5页Infection Inflammation Repair

摘　　要：目的:建立2,4-二硝基氯苯(DNCB)和醋酸(AA)复合法诱导的小鼠结肠炎模型,并验证其在炎性肠病(IBD)发病及治疗机制中的研究价值。方法:昆明种小鼠48只,按体重随机分为正常对照、模型、硫氮磺胺吡啶(SASP)及中药灌肠治疗4组,肠道积分法观察组织病理学变化;DAPI染色检测淋巴细胞凋亡指数的改变;流式细胞仪分析外周血中CD4^+CD29^+T细胞及外周血和肠系膜淋巴结中CD3^+、CD4^+ T细胞的变化。结果:成功建立免疫型小鼠结肠炎模型。模型组小鼠肠道病理学损伤较正常对照组明显加重;特别是模型组CD4^+ CD29^+ T细胞比例明显高于正常对照组(P<0.01);免疫组织淋巴细胞凋亡指数显著升高(P<0.01);而CD3^+、CD4^+ T细胞较正常对照组明显降低(P<0.05)。经SASP和中药灌肠治疗后,肠道组织病理损伤明显减轻,免疫组织淋巴细胞凋亡指数显著降低,且CD3^+、CD4^+ T细胞亚群较模型组明显增高。结论:DNCB+AA法诱导的小鼠结肠炎模型,是一种较理想的IBD动物模型,可成功用于炎性肠病发病及治疗机制的研究。Objective：To reproduce a dinitrochlorobenzene（DNCB）and acetic acid（AA）-induced murine colitis model and to assess its significance in the study of pathogenesis of inflammatory bowel disease（IBD）.Methods： Adult KM mice were randomly divided into four groups：normal control group,model group,salicylazosulfapyri- dine（SASP）treatment group and traditional Chinese medicine（TCM）treatment group.Except the normal con- trol group,ulcerative colitis was reproduced with DNCB and AA in mice of other groups.After the model was es- tablished,SASP（200 rag/L）or compound 1 TCM（1.2 g/ml）were clystered respectively in the SASP group and TCM treatment group with a dose of 0.25 mi,once per day for 1 or 2 weeks.At the same time,the same dosage of normal saline was clystered in the normal control and model groups.After 1 and 2 weeks,histopathological changes were examined and pathological score of intestinal lesion were recorded.Apoptotic lymphocytes were i- dentified with May-Grounwald-Giemsa（MGG）and proportions of CD4~＋ CD29~＋ T cell in peripheral blood and CD3~＋,CD4~＋ T cells in peripheral blood and mesenteric lymph node of each group were estimated by flow cytome- try.Results：The model of experimental colitis in mice was successfully reproduced.Compared with normal con- trol group,the pathological score was markedly higher,indicating that the pathological changes in intestine in coli- tis model group was more intensive.Especially,the proportion of CD4~＋,CD29~＋ T cell in model group was obvi ously higher than that in normal control group（P〈0.01）.The number of apoptotic lymphocytes of immune tis- sues in model group were significantly greater than those of normal control group（P〈0.01）.On the other hand, the proportions of CD3~＋ and CD4~＋ T cells were evidently decreased（P〈0.05）.After two weeks＇ treatment with SASP or TCM,the injury of intestine as estimated by pathological score was distinctively alleviated,and at the same time,apoptotitc index of lymphocyte i

关 键 词：2 4-二硝基氯苯 结肠炎 柳氮磺胺吡啶 中药灌肠 小鼠 

分 类 号：R-332[医药卫生] R574