弱精子症患者精子X，Y，18号染色体的三色荧光原位杂交分析  

An Analysis on Chromosome X,Y and 18 in the Spermatozoa of Asthenospermia Patients by Triple-color Fluorescence in Situ Hybridization

作　　者：李练兵 夏彦恺[2] 李新生 吕静 马明福 宋玲[2] 计垣 杨继高 张天枫 陈晓星 荣颖 赵乐天 

机构地区：[1]重庆市人口和计划生育科学技术研究院,重庆400020 [2]南京医科大学毒理研究所,江苏南京210029

出　　处：《中华男科学杂志》2008年第3期211-214,共4页National Journal of Andrology

基　　金：重庆市人口和计划生育委员会课题(004002)

摘　　要：目的:利用三色荧光原位杂交方法分析弱精子症患者精子X,Y,18号染色体数目畸变。方法:应用X、Y、18号染色体探针对10例弱精子症患者和5例健康男性进行三色荧光原位杂交实验,检测其精子非整倍体数目畸变情况。结果:计数45547个精子,杂交率99.18%。双体类型为XX18、XY18、YY18、X1818、Y1818。病例组各类双体率分别为(0.124±0.086)%、(0.360±0.380)%、(0.109±0.195)%、(0.342±0.746)%、(0.299±0.564)%。对照组各类双体率为(0.014±0.019)%、(0.090±0.080)%、(0.030±0.031)%、(0.068±0.103)%、(0.075±0.083)%。病例组的非整倍体率9.25%,对照组为2.70%,两者比较,差异有显著性(P(0.01)。结论:弱精子症患者的精子比对照组精子的染色体非整倍体率高,为提供更有效的科学依据,尚需大样本研究。Objective： To analyze the numerical aberration of chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization. Methods： The experiment included 10 asthenospermia patients and 5 healthy men with normal semen quality as controls. Fluorescence in situ hybridization （FISH） and probes for chromosomes including X, Y and 18 were used to determine the frequency of the aneuploid of the chromosomes in spermatozoa. Results ： Of the 45 547 spermatozoa counted from the semen samples, the hybridization rate was 99.18%. The frequencies of the chromosome disomies including XX18, XY18, YY18, X1818 and Y1818 were （0.124±0.086）% , （0.360±0.380）%, （0.109±0.195）%, （0.342±0.746）% and （0.299±0.564）% in the case group and （0.014±0.019）%, （0.090±0.080）%, （0.030±0.031）%, （0.068±0.103）% and （0.075±0.083 ） % in the control. The sperm aneuploid rate was 9.25% in the former and 2.70% in the latter, with significant difference in between （P 〈0.01 ）. Conclusion： Asthenospermia patients have a higher aneuploid rate of sperm chromosome than normal fertile men. However, larger samples are yet to be studied to obtain more scientific evidence.

关 键 词：弱精子症 性染色体 18号染色体 精子 非整倍体 染色体数目畸变 三色荧光原位杂交 

分 类 号：R698.2[医药卫生—泌尿科学]