线粒体促凋亡蛋白在膀胱移行细胞癌中的表达及其临床意义  

Expression of second mitochondria derived activator of Caspase in transitional cell cancer of bladder and the significance

作　　者：李恒[1] 廖贵益[1] 曾甫清[1] 白杨[1] 王竞[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院泌尿外科,武汉430022

出　　处：《中华实验外科杂志》2008年第3期343-345,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30271301）

摘　　要：目的检测线粒体促凋亡蛋白（Smac）在膀胱移行上皮细胞癌（TCC）中的表达并探讨其临床意义。方法逆转录-聚合酶链反应（RT-PCR）和免疫组织化学SP法分别在基因和蛋白水平检测Smac在15例正常膀胱黏膜和72例TCC中的表达。结果Smac蛋白和mRNA在正常膀胱黏膜与分化Ⅰ级TCC中均有较强表达，两者差异无统计学意义（P〉0.05），在TCC中的表达随分级的增加都显著性减少（P〈0．01，P〈0．05）。浸润性TCC中Smac蛋白和mRNA的表达都低于表浅性TCC（P〈0．01）。结论Smac在正常膀胱黏膜强表达，而在TCC中低表达，且与TCC的分级分期密切相关，检测Smac可辅助临床TCC分级、分期的判断。Objective To detect the expression of second mitochondria-derived activator of Caspase （Smac） in transitional cell cancer of bladder （TCC） and discuss its clinical significance. Methotis Smac was detected in 15 specimens of normal bladder epithelium and 72 specimens of TCC by reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry at the level of gene and protein, respectively. Results There was no statistically significant difference in the Smac protein and mRNA expression levels between normal mucous membrane of bladder and grade I TCC （P 〉 0.05）. The expression of Smac protein and mRNA in TCC was decreased gradually and significantly with the increase in grade of TCC （P 〈 0.01 and P 〈 0.05, respectively）. In invasive TCC, the expression levels of Smac protein and mRNA were higher than those of in superficial TCC （ P 〈 0.01 ）. Conclusion Normal bladder epithelium has high expression of Smac while TCC has low expression of Smac. The expression of Smac is closely related to the grade and stage of TCC. Detection of Smac expression helps to judge the grade and stage of TCC.

关 键 词：膀胱肿瘤 脱噬作用 凋亡蛋白 线粒体 

分 类 号：R737.14[医药卫生—肿瘤] R735.7[医药卫生—临床医学]