CD14、TLR4在大鼠尖周炎症组织中的表达  

The expression of CD14 and TLR4 in rat inflamed periapical tissues

作　　者：曹佳[1] 侯本祥[1] 张琛[1] 

机构地区：[1]首都医科大学口腔医学院,北京100050

出　　处：《牙体牙髓牙周病学杂志》2008年第3期130-134,共5页Chinese Journal of Conservative Dentistry

基　　金：北京市科技新星计划项目(H013610290113);首都医学发展科研基金(2005-3046)

摘　　要：目的:探讨CD14、TLR4在尖周组织中的分布和表达,为阐明CD14、TLR4在LPS引起的炎症反应中的作用机制提供实验依据。方法:分别将Pe、Pg菌液封入大鼠髓腔建立大鼠尖周炎症模型,并以髓腔自然开放和开髓后无菌坏死作为对照。免疫组织化学染色观察CD14、TLR4在尖周组织的分布情况。结果:4组的尖周炎症组织中均可见大量CD14、TLR4表达阳性细胞,以单核巨噬细胞为主。结论:LPS信号受体CD14和TLR4可能参与大鼠LPS介导的尖周炎症反应。AIM： To explore the distribution and expression of CD14 and TLR4 in periapical tissue and offer the experiment foundation for elucidating the mechanisms of CD14 and TLR4 in the inflammatory reactions induced by LPS. METHODS： The model of rat molar apical periodontitis was established by placing Porphyromonas gingivalis and Porphyrornonas endodontalis in the pulp chamber. The rats which pulp chambers were opened and were necrotic without bacterial were control groups. The distribution and expression of CD14 and TLR4 in periapical tissue were determined by immunohistochemistry technique. RESULTS： CD14 and TLR4 were strongly positively stained in inflammatory periapical tissue in all groups. Positive cells were mostly monocytes/macrophages. CONCLUSION： CD14 and TLR4 possibly participate in periapical inflammatory reactions induced by LPS.

关 键 词：尖周炎 内毒素 CDL4 TLR4 

分 类 号：R780.2[医药卫生—口腔医学]