草莓轻型黄边病毒3＇末端序列多态性研究  

Diversity of the 3' terminal sequence of Strawberry mild yellow edge virus genome

作　　者：杨洪一[1] 代红艳[2] 李丽丽[2] 张志宏[2] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]沈阳农业大学园艺学院,沈阳110161

出　　处：《植物病理学报》2008年第1期96-99,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金项目(30200187)

摘　　要：The 930 bp segment in 3’ terminal region of Strawberry mild yellow edge virus(SMYEV)genome was amplified by 3’ rapid amplification of cDNA ends(RACE).Ten Chinese isolates were sequenced,and 7 of them were the same.Nucleotide and amino acid identities and phylogenesis were analyzed between Chinese isolates and 24 isolates from other regions of the world.Sequence analysis of the 878 nt stretch within 3’ terminal region of SMYEV genome showed that nucleotide acid identities ranged from 79.5% to 100%,deduced amino acid sequences of coat protein gene identity were 86.4% to 100%.Phylogenetic analysis showed that all isolates of SMYEV fell into four clades.To a certain extent,the clades were related with the geological distribution of SMYEV.Chinese isolates SY01 and SY04 lay in the same clade with European and American isolates,but formed a small separate branch.Isolates SY03 and SY02,derived from Fragaria×ananassa cv.Changhong-2 and F.pentaphylla respectively,had a far relationship with other isolates and fell into one clade.They were likely to be the special isolates that existed only in China.The 930 bp segment in 3＇ terminal region of Strawberry mild yellow edge virus （SMYEV） genome was amplified by 3＇ rapid amplification of cDNA ends （RACE）. Ten Chinese isolates were sequenced, and 7 of them were the same. Nucleotide and amino acid identities and phylogenesis were analyzed between Chinese isolates and 24 isolates from other regions of the world. Sequence analysis of the 878 nt stretch within 3＇ terminal region of SMYEV genome showed that nucleotide acid identities ranged from 79.5% to 100%, deduced amino acid sequences of coat protein gene identity were 86.4% to 100%. Phylogenetic analysis showed that all isolates of SMYEV fell into four clades. To a certain extent, the clades were related with the geological distribution of SMYEV. Chinese isolates SY01 and SY04 lay in the same clade with European and American isolates, but formed a small separate branch. Isolates SY03 and SY02, derived from Fragaria x ananassa cv. Changhong-2 and F. pentaphylla respectively, had a far relationship with other isolates and fell into one clade. They were likely to be the special isolates that existed only in China.

关 键 词：草莓生产 序列多态性 基因组全序列 RNA病毒 virus 生物学特性 分离物 MILD 

分 类 号：S668.4[农业科学—果树学] Q987[农业科学—园艺学]