RNA干扰人端粒酶逆转录酶基因对膀胱尿路上皮癌BIU-87细胞株端粒酶活性抑制的研究  

RNA interference with the human telomerase reverse transcriptase gene inhibits telomerase activity in bladder cancer BIU-87 cell lines

作　　者：景抗震[1] 高建平[1] 程文[1] 张征宇[1] 葛京平[1] 

机构地区：[1]第二军医大学南京临床医学院(南京军区南京总医院)泌尿外科,江苏南京210002

出　　处：《医学研究生学报》2008年第3期237-241,I0001,共6页Journal of Medical Postgraduates

基　　金：江苏省博士后科研资助计划B类基金资助项目(批准号:2004B);江苏省六大人才高峰重点课题基金资助项目(批准号:2005A2)

摘　　要：目的:探讨RNA干扰技术使人端粒酶逆转录酶(hTERT)基因沉默,对膀胱尿路上皮癌BIU-87细胞株端粒酶活性的抑制作用。方法:针对hTERT基因设计构建多种siRNA表达载体,并转染至膀胱肿瘤BIU-87细胞,通过实时荧光定量、单四唑(MTT)检测端粒酶活性,观察siRNA表达载体对hTERT基因表达的影响。结果:实时荧光定量和MTT检测证明,所构建的载体能导致不同程度的hTERT基因沉默,并成功地抑制BIU-87细胞的生长。结论:RNA干扰膀胱尿路上皮癌细胞(BIU-87)端粒酶hTERT基因,能影响端粒酶基因的表达,为进一步研究端粒酶活性在膀胱尿路上皮癌中的作用奠定了基础。Objective ： To explore the inhibition of telomerase activity of bladder tumor BIU-87 cell lines by silencing the hTERT gene with the RNA interference technique. Methods： hTERT-targeted siRNA vectors were constructed and transfected to BIU-87 cells. The influence of the siRNA vectors on the expression of the hTERT gene was evaluated and the activity of telomerase was tested by Real Time Fluorescent quantization and MTT assay. Results： The siRNA vectors significantly decreased telomerase activity and inhibited cell proliferation by down-regulating ase gene expression of bladder uroepithelium cancer the hTERT expression. Conclusion ： The telomercells （BIU-87）can be influenced by silencing the hTERT gene with the RNA interference technique, which may lay a foundation for further researches on the role of telomerase activity in bladder uroepithelium cancer cell lines.

关 键 词：RNA干扰 人端粒酶逆转录酶基因 端粒酶 膀胱尿路上皮癌 

分 类 号：R737.14[医药卫生—肿瘤]