表没食子儿茶素没食子酸酯对中波紫外浅诱导的人皮肤成纤维细胞基质金属蛋白酶-1表达的影响  

Study on the photoprotection effects of epigallocatechin-3-gallate by reducing the expression of MMP-1 after UVB irradiated fiberblasts

作　　者：潘敏[1] 骆丹[1] 林向飞[1] 曹妍[1] 

机构地区：[1]南京医科大学第一附属医院皮肤性病科,江苏南京210029

出　　处：《临床皮肤科杂志》2008年第4期210-212,共3页Journal of Clinical Dermatology

基　　金：国家自然科学基金资助项目(30371294)

摘　　要：目的:研究中波紫外线(UVB)诱导人皮肤成纤维细胞(FB)表达基质金属蛋白酶(MMP)-1和表没食子儿茶素没食子酸酯(EGCG)及c-Jun氨基端激酶(JNK)抑制剂SP600125对该诱导的保护作用。方法:以30mJ/cm2UVB及12.5μg/mLEGCG处理FB,同时以SP600125为对照,照光和(或)加药后于相应时间点提取上清及总RNA,以ELISA法检测MMP-1表达,反转录(RT)-PCR法检测细胞中MMP-1mRNA含量。结果:30mJ/cm2UVB照射FB后24hMMP-1表达为对照组的3.1倍,12h及24h时MMP-1mRNA表达分别增加至对照组的2.60倍及2.66倍(P均<0.05)。UVB照射前、后加EGCG及SP600125组较单纯UVB照射组显著抑制MMP-1的表达,MMP-1mRNA含量分别为单纯照射组的71.9%及40.4%,MMP-1蛋白表达量分别为单纯照射组的61.8%及48.9%。结论:UVB照射FB后MMP-1mRNA及MMP-1表达水平均显著增加,EGCG可抑制UVB所致的MMP-1mRNA及MMP-1高表达。Objective： To explore the expression of MMP-1 after UVB irradiation on fibroblasts and the photoprotection ef leers of EGCG and JNK inhibitor. Methods： The fibroblasts were treated with 12.5 μg/mL EGCG and irradiated by 30 mJ/cm2 UVB. The conditioned supernatants and RNA of fibroblasts were extracted at propotional time points after treated with EGCG or JNK inhibtor. ELISA and RT-PCR were employed to detect the expressions of MMP-1 and MMP-1 mRNA respectively. Results： The expressions of MMP-1 protein of 24 h were increased 3.1 times, while MMP-1 mRNA of 12 h and 24 h after 30 mJ/cm2 UVB irradiation were increased 2.60 and 2.66 times respectively （P〈0.05）. MMP-1 mRNA and MMP-1 protein were decreased to 71.9%, 40.4% and 61.8%, 48.9% of the fibroblasts treated with EGCG and JNK inhibitor. Conclusion： MMP-1 mRNA was increased significantly after UVB irradiation. EGCG could decrease the upregnlation of MMP-1 mRNA and MMP-1 protein induced by UVB irradiation.

关 键 词：表没食子儿茶素没食子酸酯 紫外线 中波 成纤维细胞 基质金属蛋白酶-1 

分 类 号：R285.5[医药卫生—中药学] R339.57[医药卫生—中医学]