PV阳性神经元在孤独症模型大鼠情绪与认知相关脑区内的表达  

The expression of the PV-immunopositive neurons in the brain regions associated with emotion and cognition in animal models of autism

作　　者：王维霞[1] 王月华[1] 陈明军[1] 上野照子 小野武年 西条寿夫 李瑞锡[1] 彭裕文[1] 

机构地区：[1]复旦大学上海医学院解剖与组织胚胎学系,上海200032 [2]富山大学医科药科研究生院,系统情绪科学研究室,日本国富山930-0194

出　　处：《神经解剖学杂志》2008年第2期131-140,共10页Chinese Journal of Neuroanatomy

基　　金：教育部博士点基金(No.070246184);日本科学技术振兴机构(JST);复旦大学上海医学院基础临床交叉研究基金(No.2007-09)资助项目

摘　　要：为显示小白蛋白(Parvalbumin,PV)中间神经元在孤独症脑内的形态变化,更好地理解PV中间神经元在孤独症发病过程中的作用,本研究用免疫组化法观察了孕鼠腹腔注射丙戊酸钠(VPA)和反复冷冻刺激(RCS)法建立的两种孤独症动物模型杏仁体、前额叶皮层和海马区的PV阳性神经元的表达特点。结果显示:两种孤独症模型鼠杏仁体、前额叶皮层和海马区内的PV阳性神经元的胞体形态、大小、突起的长度和密度等与正常对照组(CTL)相比,都发生了不同程度的变化。这些结果提示,PV中间神经元在孤独症的发病过程中发挥重要的作用,它们的改变可能削弱了对孤独症相关的神经环路中锥体神经元的抑制作用。In order to reveal the morphological changes of parvalbumin （PV） intemeuron in the autistic brains for understanding the roles of the PV neurons in the autism, the immunohistochemical method was used to examine the expression of PV-immunopositive neurons in the amygdala, prefrontal cortex and hippocampus for the the offspring of pregnant rats by intraperitoneal injection of valproic acid sodium salt （VPA） and by repeated cold stress （RCS）. The results showed that the expression of PV-immunopositive neurons in the amygdala, prefrontal cortex and hippocampus of autism models changed in various degrees in shape and size of soma of the neurons and in density and length of the process of the neurons. The present results suggested that the PV-containing interneurons might play an important role in the incidence of autism, and the changed PV interneurons probably weaken their inhibition to the pyramid neurons in the brain circuits that were related to autism.

关 键 词：孤独症动物模型 小白蛋白 海马 杏仁体 前额叶皮层 大鼠 

分 类 号：R749.9[医药卫生—神经病学与精神病学]