番木瓜环斑病毒海南分离株全基因组序列分析  

Sequence Analysis of the Complete Genome of Papaya Ringspot Virus Hainan Isolate

作　　者：卢雅薇[1] 沈文涛[1] 唐清杰[1] 牛艳梅[1] 周鹏[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室

出　　处：《病毒学报》2008年第2期152-154,共3页Chinese Journal of Virology

基　　金：中央级科研院所基本科研业务费项目;国家自然科学基金(30760134)

摘　　要：Total RNA was extracted from infected papaya(Carica papaya L.) leaves in Hainan Province, and the full-length sequences of papaya ringspot virus were amplified by RT-PCR and RACE, and its complete genomic sequence was assembled,named Hainan-P isolate.The RNA genome sequence of Hainan-P isolate was 10323 nucleotides (nts)in length,excluding the 3’-terminal poly(A) tail. And it was composed of a single open reading frame encoding a polyprotein of 3343 amino acids. The result of homology analysis with twelve GenBank PRSV isolates showed that the polyprotein identity of Hainan-P ranged from 89.8% to 93.2%, that was higher than the complete nt homology of 82.3% to 89.1%. The P1 amino acid was the least conserved( sharing homology only between 65.4% and 80.1%), whereas HC-Pro,CI and CP were the most conserved. Phylogenetic tree were constructed by the Neighbor-joining method in MEGA 3.1, which showed that PRSV isolates were obviously relevant to geographical origin,and it was impossible to delineate host-specific (P type and W type)evolution.Total RNA was extracted from infected papaya （Carica papaya L. ） leaves in Hainan Province, and the full-length sequences of papaya ringspot virus were amplified by RT-PCR and RACE, and its complete genomic sequence was assembled,named Hainan-P isolate. The RNA genome sequence of Hainan-P isolate was 10323 nucleotides （nts）in length,excluding the 3＇-terminal poly（A） tail. And it was composed of a single open reading frame encoding a polyprotein of 3343 amino acids. The result of homology analysis with twelve GenBank PRSV isolates showed that the polyprotein identity of Hainan-P ranged from 89.8% to 93.2%, that was higher than the complete nt homology of 82. 3% to 89.1%. The P1 amino acid was the least conserved（ sharing homology only between 65. 4% and 80.1%）, whereas HC-Pro,CI and CP were the most conserved. Phylogenetic tree were constructed by the Neighbor-joining method in MEGA 3.1, which showed that PRSV isolates were obviously relevant to geographical origin,and it was impossible to delineate host-specific （P type and W type）evolution.

关 键 词：番木瓜环斑病毒 全基因组 同源性 系统进化树 

分 类 号：Q781[生物学—分子生物学]