菲降解菌株的分离鉴定及特性研究  

Isolation and Characterization of a Phenanthrene-Degrading Bacterial Strain

作　　者：艾芳芳[1] 曲媛媛[1] 周集体[1] 李昂[1] 关晓燕[1] 苟敏[1] 

机构地区：[1]大连理工大学环境与生命学院,大连116024

出　　处：《环境科学》2008年第4期1066-1071,共6页Environmental Science

基　　金：国家自然科学基金项目(50608011)

摘　　要：从某石化公司污水处理厂的活性污泥中分离到1株对菲具有降解活性的菌株AFF,该菌株能够以菲为唯一碳源和能源生长.根据形态特征及16S rRNA基因序列分析,该菌株初步鉴定为Burkholderiasp..在以菲为唯一碳源的培养基中,84 h内菌株对100 mg.L-1菲的降解率可达74.1%.通过液相-质谱联用及紫外可见分光光度法分析,推测该菌株代谢菲的途径为水杨酸途径.测定了降解途径中相关酶的活性,表明该菌株降解酶系中具有邻苯二酚2,3-双加氧酶活性,并证明该酶为诱导酶;确定了粗酶降解邻苯二酚的动力学常数:米氏常数Km为10.93μmol.L-1,最大反应速率Vmax为106.38μmol.min-1,说明酶与邻苯二酚的亲和力较大,邻苯二酚是该酶的良好底物.A bacterial strain that degrades phenanthrene was isolated from activated sludge of wastewater treatment plant from a chemical corporation.The strain can utilize phenanthrene as sole source of carbon and energy.It was identified as Burkholderia sp.according to morphological and 16S rRNA gene sequence analysis.Phenanthrene（100mg·L^-1） was removed 74.1% within（84 h） by this strain.It was suggested that the strain possessed the salicylic acid pathway by LC-MS and UV methods.And the strain contains an inductive catechol 2,3-dioxygenase（C23O）.The kinetic constants Km and Vmax were 10.93μmol·L^-1 and 106.38μmol· min^-1,receptively.It indicates that the affinity of the endoenzyme to catechol is great and catechol is a better substrate.

关 键 词：生物降解 菲 水杨酸途径 邻苯二酚2 3-双加氧酶 

分 类 号：X172[环境科学与工程—环境科学]