与人体β-珠蛋白基因5'旁侧正调控区(PCR)相结合的调控因子(简报)  

TRANSACTING FACTORS BINDING TO THE POSITIVE CONTROL REGION(PCR)IN THE 5' FLANKING SEQUENCE OF HUMAN β-GLOBIN GENE

作　　者：陈士友[1] 谢恒月[1] 蒋俶 钱若兰[1] 

机构地区：[1]中国科学院上海细胞生物学研究所,上海200031

出　　处：《实验生物学报》1997年第2期221-223,共3页Acta Biologiae Experimentalis Sinica

摘　　要：β-珠蛋白基因簇是研究真核基因表达时空秩序性的理想模型,在它的5个结构基因(ε,~Aγ,~Gγ,δ和β)中,成年型的β-珠蛋白基因主要在出生后人的骨髓中表达。过去的研究表明,在β-珠蛋白基因的5′旁侧序列内至少存在三个调控元件。Using gel mobility shift assay, three protein factors (Pa, Pb, PC) binding to the positive control region (PCR) in the 5' flanking sequence of human β-globin gene were detected in the nuclear extracts from mouse fetal liver at d 18 or d 19 of gestation. Competitive experiment showed that Pb and PC could bind to GATA-1 motif, therefore could be the members of GATA family. While Pa was a new and developmental stage specific trans-acting factor, we suggested that the factor Pa was related to the activation of β-globin gene.

关 键 词：基因表达 Β-珠蛋白基因 正调控区 调控因子 

分 类 号：S754[农业科学—森林经理学]