反义脱氧寡核苷酸抑制人卵巢癌HO-8910细胞株中ILK基因的表达  

The effect of antisense oligonucletide on inhibiting integirn-linked kinase gene expression in HO-8910 cell line

作　　者：李奇[1] 裴俊瑞[1] 李佩玲[2] 张艳华[2] 

机构地区：[1]哈尔滨医科大学克山病防治研究所,黑龙江哈尔滨150081 [2]哈尔滨医科大学附属二院妇产科,黑龙江哈尔滨150086

出　　处：《现代肿瘤医学》2008年第4期514-517,共4页Journal of Modern Oncology

基　　金：黑龙江省卫生厅资助项目(2006-287);哈尔滨医科大学青年科学基金资助项目(060009);哈尔滨医科大学研究生创新基金资助项目

摘　　要：目的:探讨整合素连接激酶(integirn-linked kinase,ILK)反义寡核苷酸(antisense oligonucleotide,ASODN)对卵巢癌HO-8910细胞株中ILK基因表达的抑制作用。方法:将ILK反义寡核苷酸(ILK-ASODN)导入卵巢癌细胞株,阻断ILK的表达。转染后72h,用RT-PCR和Western-bllot法检测各组卵巢癌细胞ILKmRNA和蛋白表达量的变化。结果:ILK-ASODN处理后,各组卵巢癌细胞mRNA表达量明显下降,与两对照组比较差异有统计学意义(P<0.05),且mRNA表达量随转染浓度的增高而减低;各组蛋白表达量均显著下降,各组与两对照组比较差异有极显著意义(P<0.01),且蛋白表达量随转染浓度增高而减低。结论:ILK-ASODN导入卵巢癌细胞株后可以明显抑制卵巢癌HO-8910细胞株中ILK基因的mRNA表达和蛋白表达。Objective： To explore the effect of integirn - linked kinase - antisense oligonucletide （ ILK - ASODN） on inhibiting ILK gene expression in human ovarian cancer cell lines （HO -8910）. Methods： We used lipofectamine 2000 to transfect ILK - SODN and ILK - ASODN into HO - 8910 to block ILK expression. The experiment had six groups, blank control （A）, lipofectamine 2000 control （B）, ILK-SODN 100nmol/L （C）, and three ILK - ASODN groups of 60nmol/L （D） , 80nmol/L （E） , and 100nmol/L （F）. 72h after transfection, we measured the expression levels of ILK mRNA by RT - PCR and ILK protein by Western - blotting. Results： After transfection of ILK- ASODN, the expression levels of mRNA decreased significantly in the experimental groups; group D showed significance （P〈0.05） and group E and F showed extremely significance （P 〈0.01） in comparison with the two control groups ; and the expression levels of mRNA decreased as the transfection levels increased. After ILK - SODN transfection, the expression levels of mRNA did not change significantly in group C （ P 〉 0.05 ）. After transfection of ILK - ASODN, the expression levels of protein decreased significantly in group D, E, F （ P 〈 0.01 ） in comparison with the two control groups ; the expression levels of protein decreased as the transfection levels increased. After ILK - SODN transfection, the expression levels of protein decreased significantly in group C （ P 〈 0.05 ）. Conclusion： Transfection of ILK - ASODN into human ovarian cancer line inhibited ILK gene and protein expression in HO - 8910.

关 键 词：整合素连接激酶 反义脱氧寡核苷酸 卵巢癌细胞株 蛋白表达 

分 类 号：R73-3[医药卫生—肿瘤] R737.31[医药卫生—临床医学]