甲状腺乳头状癌组织PTTG及PTTG mRNA表达研究  

Significance of PTTG and PTTG mRNA in papillary thyroid carcinoma

作　　者：梁华晟[1] 钟宇华[1] 

机构地区：[1]广西医科大学第九附属医院内分泌科,广西北海536000

出　　处：《现代肿瘤医学》2008年第4期535-536,共2页Journal of Modern Oncology

基　　金：广西科技厅自然科学基金资助项目(桂科No.0339080);广西卫生厅自筹经费科研课题(Z007003);广西科技厅青年科技基金资助项目(桂青科0728108)

摘　　要：目的:检测PTTG在甲状腺乳头状癌组织中的表达,探讨其与甲状腺乳头状癌的关系。方法:在52例甲状腺乳头状癌组织及其癌旁组织中分别应用原位细胞凋亡检测(TuNEL)试剂盒测定原位细胞凋亡指数(apoptosis index,AI),应用免疫组化方法检测PTTG,应用原位杂交方法检测PTTG mRNA表达。结果:PTTG及PTTG mRNA在甲状腺乳头状癌组织中表达比相应癌旁组织显著升高(P=0.0091,P=0.0012):PTTG及PTTG mRNA灰度值与AI正相关(r=0.447,P=0.003;r=0.564,P=0.000)。结论:PTTG及PTTG mRNA表达升高可能参与甲状腺乳头状癌的发生发展,检测PTTG及PTTG mRNA对于甲状腺乳头状癌与良性组织鉴别诊断具有临床参考意义。Objective：To study the relationship between PTTG and papillary thyroid carcinoma by combining assays of PTTG and PTTG mRNA in papillary thyroid tissue. Methods： Apoptosis index was evaluated by TUMEL method, the expression of PTTG and PTTG mRNA were detected by immunohistochemistry and in situ hybridization in 52 papillary thyroid carcinoma cases and tissue around carcinoma. Results： The higher level of PTTG and PTTG mRNA in papillary thyroid carcinoma was found compared with tissue around carcinoma（ P = 0. 0091, P = 0.0012 ） ; There had positive relationship between the expression of PTTG, PTTG mRNA and AI （ r = 0. 447, P = 0. 003 ; r = 0. 564, P = 0. 000 ）. Conclusion： PTTG may participate in proceeding of papillary thyroid carcinoma, it is sug- gested that combined detections of PTTG and PTTG mRNA may have important reference significance on the differentiation of benign and malignant papillary thyroid diseases.

关 键 词：甲状腺乳头状癌 细胞凋亡 PTTG 

分 类 号：R736.1[医药卫生—肿瘤]