非小细胞肺癌患者外周血CD_4^+CD_(25)^+调节T细胞的检测及其临床意义  

CD_4^+CD_(25)^+ regulatory T cells in peripheral blood from patients with non small cell lung cancer

作　　者：肖莉[1] 

机构地区：[1]武汉科技大学附属医院呼吸内科,430064

出　　处：《疑难病杂志》2008年第4期204-206,共3页Chinese Journal of Difficult and Complicated Cases

摘　　要：目的探讨非小细胞肺癌(NSCLC)患者外周血CD_4^+CD_(25)^+调节T细胞(Treg)水平的变化特点及其临床意义。方法采用流式细胞仪检测70例NSCLC患者和30例健康人外周血CD_4^+CD_(25)^+Treg水平,并进行2组水平比较和分层分析。结果NSCLC组外周血CD_4^+CD_(25)^+Treg水平(29±5)%,明显高于健康对照组(11±5)%(P<0.01);外周血CD_4^+CD_(25)^+Treg水平与NSCLC病理类型无相关性(P>0.05);与临床分期密切相关,I+II期亚组(16±5)%与健康对照组比较差异无统计学意义(P>0.05),而与III期亚组(26±6)%、IV期亚组(35±6)%比较差异有统计学意义(P<0.01)。结论NSCLC患者外周血CD_4^+CD_(25)^+Treg水平明显升高,且与肿瘤的发生发展密切相关,可作为评估肺癌患者预后的一项指标。Objective To investigate the character of CD4^＋ CD25^＋ -regulatory T cells （Treg） in peripheral blood in patients with non small cell lung cancer （NSCLC）, and discuss its clinical significance. Methods Colleeted the peripheral plasma from 70 cases of NSCLC and 30 cases of normal control, and detected the level of CD4^＋ CD25^＋ Treg with flow cytometry. The data was analyzed and compared between two groups. Results The NSCLC patients showed an obviously increasement of CD4^＋ CD25^＋ Treg came from the peripheral blood, compared with normal control group [（29±5）% vs （11±5）%（ P 〈0.01）] .There was no relation between CD4^＋ CD25^＋ Treg and pathological classification, but was closely related with clinical stage. There was no statistical difference between stage Ⅰ ＋ Ⅱ subgroup （ 16 ± 5）% and normal control group（ P 〉 0.05）, but did obviously difference compared with stage Ⅲ subgroup（26 ± 6） % and stage Ⅳ subgroup（35 ± 6） %, （ P 〈 0.01 ）. Conclusion It had been proved that the level of CD4^＋ CD25^＋ Treg in the peripheral blood was higher in the cases of NSCLC, and the CD4^＋ CD25^＋ Treg was related with the regression of tumor. It could be one of important indexes to evaluate the progression of NSCLC.

关 键 词：肺癌 非小细胞 CD4^+CD25^+调节T细胞 流式细胞技术 

分 类 号：R734.2[医药卫生—肿瘤]