高效菲降解菌的分离鉴定及细胞表面结构研究  

Screening of a phenanthrene-degrading bacterium and its microstructure of cell surface

作　　者：赵渝[1,2] 高林[3] 徐亚同[1] 郭鲁申[3] 陆贻通[4] 

机构地区：[1]华东师范大学资源与环境科学学院 [2]上海师范大学生命与环境科学学院,上海200234 [3]上海师范大学生命与环境科学学院 [4]上海交通大学资源与环境系

出　　处：《华东师范大学学报（自然科学版）》2008年第2期92-99,共8页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金(200337010);国家基础研究发展计划973(2004CB418503);上海市基础研究重点项目(04JC14051)

摘　　要：从东北老工业基地长期受石油与重金属污染的土壤中,分离获得了一株以菲为唯一碳源的高效降解菌株ZX16,通过形态、生理生化和分子生物学鉴定为溶芳烃鞘氨醇单胞菌(Sphin- gomonas aromaticivorans).菌株降解菲的特性研究结果表明,ZX16可耐受2 500 mg/L的菲,对1500 mg/L的菲在72h内降解率为98.2%.不同的重金属离子对菌株的降解效能影响大小依次为Cu^(2+)>Cd^(2+)>Zn^(2+)>Pb^(2+).菌株在中性条件下生长良好,原子力显微镜观察结果表明,菌株在复合污染物胁迫下细菌体积和形态有显著变化,细胞表面结构也从粗糙趋向光滑.One strain which could use phenanthrene as sole carbon source was isolated from petroleum and heavy metal containminated soil,collected from old industry depot in northeast China. Based on morphological and physio-biochemical characteristics and homology identification of 16S rDNA sequence, the strain ZX16 was identified as Sphingomonas aromaticivorans. In the mineral salts medium under initial phenanthrene concentration of 1 500 mg/L the removal rates of phenanthrene was 98.2% within 72 h. Tolerance of ZX16 to phenanthrene exceeds 2 500 mg/L and ZX16 was relatively to the cell growth at neutral pH value. The effect of different heavy metal on degrading rate was Cu^2＋〉Cd^2＋〉Zn^2＋〉Pb^2＋ in order. Microstructure of cell surface screening by Atomic Force Microscopy shows that the volume and morphous of strain have significant changes and the surface also changes from rough to smooth and glossy.

关 键 词：降解 菲 复合污染 原子力显微镜 

分 类 号：X172[环境科学与工程—环境科学]