阔瓣含笑叶片总酚超声波提取工艺的优化  

Optimization on Ultrasonic Extraction Technology of Total Phenols from the Michelia platypetala Hand Leaves

作　　者：毕慧敏[1] 何开跃[1] 李晓储[2] 黄利斌[2] 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037 [2]江苏省林业科学院

出　　处：《林业科技开发》2008年第2期26-29,共4页China Forestry Science and Technology

基　　金：江苏省农业三项工程子项目(编号:SX2003-77)

摘　　要：以阔瓣含笑叶片为原料,定性鉴定了其提取物中的酚类物质,并对酚类物质的超声波提取工艺进行了优化,采用单因素实验法对影响总酚提取率的4个因素(乙醇浓度、料液比、提取温度和提取时间)进行了分析,利用正交试验法确定了提取总酚的最佳工艺条件。结果表明,提取液具有典型的酚类化合物特性,定性反应现象明显;阔瓣含笑叶片中总酚的最佳提取条件为:乙醇浓度80%,料液比1∶40,提取温度55℃,提取时间30 min。在最佳提取条件下的总酚提取率为10.57%。Phenols in the extracts of M. plat3petala were analyzed qualitatively in this paper. The effects of four factors （ethanol concentration, ratio of material to solvent, extraction temperature and extraction time） on the extraction ratio of to- tal phenols from M. platypetala leaves were analyzed by single factor experiment, and the optimum extraction technology of total phenols was also identified by the orthogonal experimental design. The results showed that the typical specificity of the phenols was found in the extracts. The optimal conditions for the extraction of total phenols from M. platypetala leaves were as： ethanol concentration 80% and ratio of material to solvent 1：40 at the 55℃ of extraction temperature for 30 min, by which 10.57% of total phenols was extracted.

关 键 词：阔瓣含笑 总酚 提取工艺 

分 类 号：S688[农业科学—观赏园艺] TS972.1[农业科学—园艺学]