毛白杨无性系85号高效再生系统的建立  

Establishment of an Efficient Regeneration System for Populus tomentosa Clone-85

作　　者：李科友[1] 朱海兰[1] 赵忠[1] 樊军锋[1] 李玲[2] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100 [2]中国林业科学研究院林业研究所

出　　处：《林业科技开发》2008年第2期45-48,共4页China Forestry Science and Technology

基　　金：科技部国家转基因植物研究与产业化专项“耐盐抗虫转基因杨树新品种选育”(编号:JY03-B-26-02)

摘　　要：应用均匀设计和正交试验筛选出毛白杨无性系85号叶片不定芽诱导最佳组合为MS+0.50 mg/L 6-BA+0.01 mg/L NAA,诱导不定芽生根最适宜的培养基及激素配比为1/2MS+0.50 mg/L NAA+0.05 mg/L IBA。此条件下,叶片平均产生不定芽21.2个,不定芽生根率可达到99%,生根数为7条。该研究为毛白杨无性系85号工厂化育苗和利用叶盘法开展基因转化培育抗逆新品种奠定了良好基础。To optimize the regeneration system of P. tomentosa Clone-85, influences of the different medium and proportion of hormone on adventitious bud and root for leaf-explants were studied by uniform design and orthogonal experimental design. The results demonstrated that the optimal medium and proportion of hormone was MS ＋0. 5 mg/L 6-BA ＋0. 01 mg/L NAA for bud differentiation, andl/2MS＋0. 05 mg/L NAA＋0. 05 mg/L IBA for adventitious root. Using the optimal regeneration system of P. tomentosa Clone-85, the average 21.2 buds was induced in each leaf, the rooting rate was 99%, and the average root number was 7. The results laid a sound foundation for regeneration of P. tomentosa 85 in industry and gene transformation by leaf disc method.

关 键 词：毛白杨无性系85号 植株再生 组织培养 

分 类 号：S792.11[农业科学—林木遗传育种] S651[农业科学—林学]