非酒精性脂肪性肝病发生中Perilipin和ADRP表达变化实验研究  

Analysis of Perilipin and ADRP changes in the nonalcoholic fatty liver disease

作　　者：朱颖 陈东风[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所消化内科,重庆400042

出　　处：《西南国防医药》2008年第2期169-171,F0004,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金(2005CB522602)

摘　　要：目的:探讨高脂饮食诱导大鼠非酒精性脂肪性肝病(NAFLD)形成过程中,肝组织Perilipin(脂滴包被蛋白)和ADRP(脂肪分化相关蛋白)的表达变化及意义。方法:建立大鼠高脂饮食NAFLD模型并设正常对照组,观察时间点为建模后4w、8w和12w,观察大鼠肝组织病理变化,检测血浆游离脂肪酸及甘油三酯水平,免疫组织化学染色法检测肝组织中Perilipin和ADRP蛋白水平。结果:成功制作大鼠NAFLD实验模型,与正常组相比,NAFLD组大鼠血浆FFA水平和TG水平从第4w起显著升高(P<0.01),NAFLD组大鼠肝组织Perilipin蛋白表达水平显著低于正常组(P<0.01),ADRP蛋白表达水平显著高于同期正常组(P<0.01)。Perilipin及ADRP表达与FFA及TG具有显著相关性。结论:高脂饮食可制作NAFLD大鼠模型,Perilipin和ADRP与NAFLD的形成有关。Objective：To investigate the changes of Perilipin and ADRP in the development of nonalcoholic fatty liver disease. Methods： Rat model of nonalcoholic fatty liver disease was established with fatty rich diet. The expression of Perilipin and ADRP in the rat＇s liver was dynamically detected by immunohistochemistry stainning at the 4th, 8th and 12th week. Meanwhile, FFA and TG levels in serum were detected. Results：Compared with the normal group, the serum levels of FFA and TG in NAFLD group increased significantly from beginning of the 4th week（P〈0. 01）. The expression of Perilipin decreased significantly from the 4th week to 12th week, but the expression of ADRP increased significantly from beginning of the 4th week（P〈0. 01）. The changes of Perilipin and ADRP were correlated with FFA and TG. Conclusion： High fat diet can be used to induce successfully NAFLD in rats. Perilipin and ADRP are involved in the formation of NAFLD.

关 键 词：非酒精性脂肪性肝病 游离脂肪酸 甘油三酯 脂滴包被蛋白 脂肪分化相关蛋白 

分 类 号：R575.5[医药卫生—消化系统] R774.13[医药卫生—内科学]