鼻咽癌患者血清中自身抗体的初步检测  

Detection of autoantibodies in the sera of nasopharyngeal carcinoma patients

作　　者：童永清[1] 黄建[1] 周国华[1] 李跃辉[1] 胡锦跃[1] 李官成[1] 

机构地区：[1]中南大学肿瘤研究所,长沙410078

出　　处：《中南大学学报（医学版）》2008年第3期233-237,共5页Journal of Central South University ：Medical Science

基　　金：中南大学博士生创新基金(2340-76235)~~

摘　　要：目的:探讨鼻咽癌患者血清中是否存在抗鼻咽癌自身抗原的抗体,为寻找鼻咽癌新的肿瘤标志物提供依据。方法:制备Epstein-Barr病毒阴性的鼻咽癌细胞株CNE1细胞板,酶联免疫吸附实验(ELISA)分析其与32例鼻咽癌患者血清和54例正常血清反应的差异性;提取CNE1总蛋白,Western印迹分析其与鼻咽癌患者血清反应是否存在特异性的蛋白质。结果:ELISA分析显示鼻咽癌患者血清平均滴度(0.904±0.032)明显高于正常人血清平均滴度(0.736±0.028)(P<0.01);Western印迹分析发现鼻咽癌患者血清与正常人血清相比,部分阳性条带一致,但条带强度增加,还发现出现了部分新的阳性条带,这些条带可能属于鼻咽癌相关抗原或鼻咽癌特异性抗原。结论:鼻咽癌患者血清中存在抗鼻咽癌自身抗原的抗体,这些抗体并非针对Epstein-Barr病毒,为寻找鼻咽癌血清肿瘤标志物提供了依据。Objective To investigate whether there are autoantibodies to nasopharyngeal carcinoma （ NPC ） in the sera of patients and to find new NPC biomarkers. Methods Cell plate of Epstein Barr virus negative NPC cell line CNE1 was prepared, and difference between 32 NPC patient sera and 54 normal sera was analyzed by ELISA. We extracted the total protein of CNE 1 , and analyzed whether there were specific proteins to react with NPC sera by Western blot. Results The average absorbance values of serum antibody in NPC patients （ 0.904±0. 032 ） were significantly higher than those of normal serum antibody absorbance values （ 0. 736±0. 028） （ P 〈 0.01 ）. The analysis with Western blot showed there were positive bands, and some of these were unanimous bands, but the intensity increased, and some of these were new bands compared with the normal sera. These positive bands may be NPC tumor-associated antigens or NPC tumor-specific antigens. Conclusion Autoantibodies that react with NPC exist in the sera of NPC patients, but they do not react with Epstein-Barr virus. It provides the basis to seek the tumor biomarkers in NPC sera.

关 键 词：鼻咽癌 肿瘤标志物 自身抗体 

分 类 号：R739.63[医药卫生—肿瘤]