运动干预对高脂喂养大鼠脂肪组织内脏脂肪素表达的影响  

Effect of exercise on expression of visfatin of visceral fat in high-fat-diet-fed rats

作　　者：尚敬[1] 陈璐璐[1] 孙晖[1] 肖方喜[1] 舒砚文[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院内分泌科,湖北武汉430022

出　　处：《中国现代医学杂志》2008年第5期551-554,共4页China Journal of Modern Medicine

基　　金：湖北省科技攻关基金(No:2005AA30C133)

摘　　要：目的研究运动对高脂喂养大鼠内脏脂肪素(visfatin)基因表达的影响,以探讨visfatin与内脏型肥胖、代谢紊乱的联系。方法健康Wistar大鼠30只随机分为高脂饲料喂养组(HD)和正常饲料喂养组(ND),18周后,HD组再随机分出一半大鼠为高脂喂养游泳组(HS),HS组进行游泳运动6周后,测定大鼠体重、体长、血脂、空腹胰岛素和血糖等,用正糖-高胰岛素钳夹术检测部分大鼠胰岛素敏感性。RT-PCR检测各组大鼠肾周脂肪visfatin基因表达量。结果第18周时HD组大鼠Lee's指数、血糖、血脂明显高于ND组;第24周时,与HD组相比,HS组大鼠血脂、胰岛素敏感性等得到明显改善,HS组大鼠内脏脂肪量明显减少(P<0.01),同时HS组肾周脂肪内visfatin基因表达下降(P<0.01)。结论visfatin的表达与内脏脂肪量、代谢紊乱有密切联系;运动可改善高脂喂养所导致的代谢紊乱和肥胖,其作用可能与visfatin的表达有关。[Objective] To investigate the effect of exercise on rats fed with high fat and explore the relationship between visfatin and visceral obesity, metabolic dysregulation. [Methods] 30 healthy Wistar rats were randomly assigned to normal-fat-diet group （ND） and high-fit-diet group （HD）. 18 weeks later, half rats of the HD group were randomly assigned to high-diet swimming grou（HS）. The HS group was arranged to swim for 6 weeks. Then body weight and body length of rats, blood lipid, fasting blood glucose and insulin were measured. Half rats of each group received insulin sensitivity with Hyperinsulinemic-euglycemic clamp estimation . The visfatin gene expression was measured by RT-PCR. [Results] In the 18th week, the Lee＇s index, fasting blood glucose and insulin, blood lipid of HD group were significantly higher than those of ND group; In the 24th week, metabolic indexes of HS group such as hyperlipidemia, insulin sensitivity were markedly improved and the visceral fat deposit was significantly reduced comparing with HD group（P 〈0.01）. Meanwhile, the gene expression of visfatin in perirenal fat in HS rats declined correspondingly compared with HD group. [Conclusion] There is close relationship between the expression of visfatin and the visceral fat deposit, metabolic dysregulation, Weight lose by exercise is partly due to the inhibition of visfatin in visceral fat .

关 键 词：内脏脂肪素 运动 内脏型肥胖 代谢综合征 

分 类 号：R-332[医药卫生]