血清双向凝胶电泳技术的建立和优化  

Establishment a two-dimensional polyacrylamide gel electrophoresis of serum

作　　者：廖秋林[1] 陈晓东[1] 赵亮[2] 丁彦青[2] 

机构地区：[1]广州军区广州总医院病理科,广东广州510010 [2]南方医科大学病理学教研室,(广东省分子肿瘤病理学重点实验室),广东广州510515

出　　处：《中国现代医学杂志》2008年第5期575-578,共4页China Journal of Modern Medicine

基　　金：国家基础研究重大发展计划(973)项目子课题(No:2005AA21610);广东省科技攻关重大专项(No:2003A308401)

摘　　要：目的建立起较好及较稳定的的血清双向凝胶电泳技术。方法对血清双向凝胶电泳中血清标本的收集、保存,血清高丰度蛋白的去除,电泳条件,上样量,IPG干胶条的选择及缓冲液的配制等多种条件和技术方法进行调整和优化。结果建立了较稳定和较好重复性的血清双向凝胶电泳技术,分辨率得到一定的提高,有效分离的蛋白质点明显增多,许多低丰度蛋白被分离出来。结论通过不断的摸索和验证,可以建立起较好的血清双向凝胶电泳技术,为进一步的分析、鉴定和寻找与疾病相关的血清蛋白质奠定一定基础。[Objective] To establish a good two-dimensional polyacrylamide gel electrophoresis （2-DE） technique for serum proteomics research. [Methods] Various conditions of 2-DE of serum were adjusted and optimized, such as how to collect and save serum sample, how to depletion high abundance proteins of serum, how to control the conditions of electrophoresis and the quantum of serum sample, and how to select IPG dry strip and confect buffer solution. [Results] A steady and high repetition 2-DE technique which can enhance resolution effective was established. And more protein dots especially some low abundance proteins were separate. [Conclusion] A good method of 2-DE technique for serum is established after long explorations and test, witch can be effectively applied in serum proteomics research.

关 键 词：血清蛋白质组学 双向凝胶电泳 白蛋白 IGG 

分 类 号：R36-33[医药卫生—病理学]