胆囊收缩素八肽拮抗谷氨酸神经毒性作用的病理研究  

The pathologic study on cholecystokinin-octapeptide on improving the neuronal damage glutamate- induced in the cerebral cortex and hippocampus CA_1 region neurons.

作　　者：梁英武[1] 张国荣[1] 单巍松[1] 王朝辉 吴希如[1] 

机构地区：[1]北京医科大学第一医院儿科神经组,山东济宁医学院儿科教研室272115

出　　处：《中国神经精神疾病杂志》1997年第4期210-212,共3页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科学基金资助项目 (批准编号 :39370 2 5 9)

摘　　要：目的　观察胆囊收缩素八肽 (CCK -8)对谷氨酸神经毒性作用所致神经元病理损害的影响。　　方法　采用扫描及透射电镜技术分别观察经不同因素处理后的原代培养的大鼠大脑皮层神经元和经侧脑室注射药物后的大鼠海马CA1 区神经元的形态学变化 ,同时应用生物体视学计量分析法对细胞内线粒体各指标的变化进行比较。　　结果　CCK -8在体内外均能明显改善谷氨酸所致神经元胞体、细胞膜肿胀及破溃 ,轴、树突断裂、丢失或线粒体肿胀、数目减少等病理损害。　　结论　CCKObjective To investigate the effect of cholecystokinin octapeptide (CCK-8) on improving the neuronal damage glutamate induced in the cerebral cortex and hippocampus CA 1 region neurons. Methods The cytomorphological changes of cultured and hippocampus CA 1 region neurons which were treated by various factors were observed with scanning and transmission electron microscopy, and various indexes of mitochondria in hippocampus CA 1 region neurons were examined with biogenetic sterotogy analysis. Results CCK-8 can significantly attenuate destrution of neuronal structure induced by glutamate including cell body swelled, cell membrance brought down dendritic and axonal swelled or lost both in vivo and in vitro. Conclusions The CCK- 8 may prevent neuronal damage from glutamate induced neurotoxicity.

关 键 词：胆囊收缩素八肽 谷氨酸 神经毒素 惊厥 

分 类 号：R362[医药卫生—病理学] R720.597[医药卫生—基础医学]