氦-氖激光对离体宫颈癌细胞增殖的影响  

Effect of He-Ne laser irradiation on proliferation of HeLa cells of human cervix cancer in vitro

作　　者：张文庚[1] 王艳萍[1] 陈晓禾[1] 徐刚[1] 陈传真[2] 韩洪富[2] 

机构地区：[1]华西医科大学肿瘤研究所,成都610041 [2]华西医科大学附属第一医院激光科

出　　处：《中华理疗杂志》1997年第3期137-139,共3页Chinese Journal of Physical Therapy

基　　金：国家自然科学基金资助

摘　　要：目的:全面认识氦-氖激光的生物效应。方法:分别用17.27J/cm^2,34.54J/cm^2,69J/cm^2剂量的氦-氖激光照射人宫颈癌 HeLa 细胞,隔天1次,共6次。照射结束后48,72,96小时对8组细胞进行增殖实验分析。结果:3组细胞增殖加快(P<0.05),1组降低(P<0.01),4组元差异(P>0.1),所有数据经秩和检验表明,氦-氖激光照射对细胞增殖无明显影响(u=1.6158,P>0.1)。在照射结束后立即进行的克隆形成分析实验中,与对照相比差异亦无显著性(P>0.05)。结论:氦-氖激光对宫颈癌带瘤患者的照射可能是安全的。Objective:To gain an insight into the biological effects of He-Ne laser on HeLa cells. Methods:The HeLa cells in vitro were separately treated by low-energy He-Ne laser with different doses (17.27J/cm^2,34.5J/cm^2 and 69J/cm^2),which was once an every other day for 6 times.At the 48,72, 96hrs of post-radiation,the MTT colorimetric assay and colony formation test were immediately per- formed in the HeLa cells oF eight groups.Results:The rapid cell proliferation was seen in three groups,the slow-down in one group;another four groups were not difference.The statistical data proved that He- Ne laser irradiation hadn't stimulated or inhibited the cell proliferation.On the colony formation analysis, there wasn't difference compared to control group(P>0.05).Conclusion:It is suggested that the He-Ne laser is a safe and effective therapy for treating the cervix cancer patients.

关 键 词：氦-氖激光 宫颈肿瘤 细胞增殖 激光疗法 

分 类 号：R737.330.5[医药卫生—肿瘤]