层粘连蛋白总糖肽增强巨噬细胞肿瘤杀伤功能的机制探讨  

Exploration of the LN Gps upregulating effect on macrophage cytotoxicity against B16 MBK cells

作　　者：刘艺萍[1,2] 周柔丽[1,2] 张莎 

机构地区：[1]第二军医大学基础医学部生物学教研室 [2]北京医科大学细胞生物学教研室

出　　处：《第二军医大学学报》1997年第3期222-224,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：探讨层粘连蛋白总糖肽（ＬＮ－Ｇｐｓ）增强巨噬细胞（Ｍφ）杀伤黑色素瘤Ｂ１６－ＭＢＫ细胞作用的机制。方法：将细菌脂多糖（ＬＰＳ，５ＥＵ／ｍｌ），甘露聚糖（Ｍａｎｎａｎ，１０ｍｇ／ｍｌ），ＬＮ－Ｇｐｓ（２００μｇ／ｍｌ）及ＰＢＳ（对照），１００μｌ／孔，分别刺激小鼠腹腔Ｍφ，并于持续刺激６，８，１６，２４，３２，４０ｈ后观察Ｍφ对Ｂ１６细胞的直接杀伤作用及其条件培养基对Ｂ１６细胞的杀伤作用。结果：Ｍφ经各种刺激作用１６ｈ后，对Ｂ１６细胞总的杀伤率各组无显著差异；各组在刺激后各时间里Ｍφ对Ｂ１６细胞的直接杀伤率的动态变化相似。但各种刺激时间后收集的条件培养基对Ｂ１６细胞的杀伤率动态曲线明显不同，ＬＰＳ及Ｍａｎｎａｎ刺激后，以６ｈ的条件培养基杀伤作用最强（Ｐ＜０．０５），８ｈ后回到对照水平，而ＬＮ－Ｇｐｓ组则在刺激１６ｈ后逐渐增强，４０ｈ达到高峰。结论：ＬＮ－Ｇｐｓ可刺激Ｍφ分泌一些可溶性物质杀伤Ｂ１６细胞，此作用机制与其所含高甘露糖型糖链结构无关，与ＬＰＳ的作用也明显不同。Objective:To explore the mechanisms of LN Gps upregulating effect on macrophage cytotoxicity against B16 MBK cells. Methods: Macrophages, collected from BALB /c mouse peritoneal lumen, were stimulated with LPS(5 EU /ml), Mannan(10 mg /ml), LN Gps(200 μg /ml) and PBS (control) for 6,8,16,24,32,40 h and their direct cytotoxicity to B16 target cells and the conditioned media extracellular cytolysis of B16 cells were measured using MTT colorimetric methods. Results: After 16 hours' stimulation, the total cytotoxicity rates in all groups were not significantly varied, and the contacting direct cytotoxicity to B16 target cells was similar in the continuous observation time in every group. But, for the conditioned media, the effects were quite different: The conditioned media collected after 6 hours' stimulation with LPS and Mannan, showed the most potency in killing B16 cells, while with LN Gps, as the stimulation time prolonged, the cytolysis rates of the conditioned media elevated, showing the greatest increase after 40 hours' stimulation. Conclusion: LN Gps can stimulate macrophages to secrete some soluable materials in the media to kill the B16 cells, the effect is not related to the high mannose structure in the LN Gps, and is not related to LPS either.

关 键 词：层粘连蛋白 糖肽 巨噬细胞 肿瘤细胞 杀伤 

分 类 号：R730.51[医药卫生—肿瘤]