肿瘤坏死因子抗人鼻咽癌细胞的活性  

Tumor necrosis factor α activity against human nasopharyngeal carcinoma

作　　者：吴玮[1,2,3] 周水淼[1,2,3] 李兆基 杨如俊[1,2,3] 何晓龙 

机构地区：[1]第二军医大学长海医院耳鼻咽喉科 [2]第二军医大学海军医学系放射医学教研室 [3]第二军医大学基础医学部神经生物学教研室

出　　处：《第二军医大学学报》1997年第3期266-268,共3页Academic Journal of Second Military Medical University

摘　　要：目的：研究新型重组人肿瘤坏死因子α（ＮｒｈＴＮＦ－α）对鼻咽癌细胞株（ＣＮＥ－２）的细胞致死性和联合用药的协同作用。方法：用细胞毒分析法观察了ＮｒｈＴＮＦ－α、重组人肿瘤坏死因子α（ｒｈＴＮＦ－α）对ＣＮＥ－２生长的影响，并对不同联合用药模式进行研究。结果：ＮｒｈＴＮＦ－α对ＣＮＥ－２细胞的杀伤作用明显强于ｒｈＴＮＦ－α，同一浓度的杀伤作用强２～３倍。细胞杀伤效应为剂量依赖型，ＮｒｈＴＮＦ－α与重组人干扰素γ（ｒｈＩＦＮ－γ）联合有较好的协同作用；再与顺铂（ＤＤＰ）联合应用则协同作用更强。结论：ＮｒｈＴＮＦ－α对ＣＮＥ－２细胞有很强的细胞杀伤性，ｒｈＩＦＮ－γ和ＤＤＰ可明显增强ＮｒｈＴＮＦ－α的细胞毒性。Objective:To study the cell lethality of new recombinant human tumor necrosis factor α(NrhTNF α) against CNE 2 nasopharyngeal squmous carcinoma cell and synergistic effect of combined drugs.Methods : This study examined the effects of NrhTNF α, recombinant human tumor necrosis factor α(rhTNF α), recombinant human interferon γ(rhIFN γ),and the combined NrhTNF α+rhIFN γ preparation with or without cisplatin (DDP) on CNE 2 cell line using the MTT assay. Results: Cell killing effects of 72 h exposure to NrhTNF α were 2 3 fold more than to rhTNF α. Cell killing effects were dose related. Combination of NrhTNF α+rhIFN γ produced synergistic cell killing on CNE 2 cell line. Additional experiments showed better effect in combination with DDP.Conclusion: These results suggest that NrhTNF α has a significant cell lethality against CNE 2 cell line and rhINF γ and DDP appear to have enhanced cytotoxic activity of NrhTNF α.

关 键 词：鼻肿瘤 肿瘤坏死因子Α 干扰素Γ 药物疗法 

分 类 号：R739.630.5[医药卫生—肿瘤]