柱切换HPLC法直接测定人尿中咖啡因的含量  

Determination of Caffeine in Human Urine by Column Switching HPLC

作　　者：朱绍棠 杨树民 王小兵 

机构地区：[1]国家体委运动医学研究所

出　　处：《药物分析杂志》1997年第5期329-331,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：用柱切换高效液相系统直接进样测定人尿中咖啡因的含量，尿样中咖啡因的回收率大于９８％，最低检测浓度可达到０．２ｍｇ／Ｌ。同时与液相提取测定咖啡因的方法进行了比较，结果表明，直接进样法测定咖啡因不仅方法简便，而且减少了提取过程所造成的损失，使定量结果更加准确可靠。A HPLC method with column switching has been developed for detection of caffeine in human urine.The urine sample was directly injected into a precolumn after 3 5min,column switch,the concentrated caffeine was eluted into an analytical column.Caffeine was detected by a diode-array detector at 275nm.The recoveries of the method were above 98%.The relative standard deviation was 0 21%～0 64%.The detection limit was 0 2mg/L.The method is sensitive enough for doping analysis and has been successfully used in the doping control.

关 键 词：高效液相色谱 咖啡因 柱切换 兴奋剂检测 HPLC 

分 类 号：R927.2[医药卫生—药学] R971.7