光线性角化病皮损细胞Bcl-2基因表达产物的定量分析  

Quantitative analysis of Bcl 2 gene expression product in cells of cutaneous lesions of actinic keratosis

作　　者：李玉平[1,2,3,4] 廖海鹰[1,2,3,4] 王凤荣[1,2,3,4] 孙修蓉[1,2,3,4] 四荣联[1,2,3,4] 张玉敏[1,2,3,4] 姚春华[1,2,3,4] 张贵贞[1,2,3,4] 

机构地区：[1]河北医科大学第二医院皮肤科 [2]河北医科大学第二医院外科 [3]河北医科大学基础所病理室 [4]河北医科大学第二医院病理科

出　　处：《临床皮肤科杂志》1997年第4期225-227,共3页Journal of Clinical Dermatology

摘　　要：Ｂｃｌ－２蛋白是Ｂｃｌ－２基因的表达产物，可抑制细胞凋亡，在某些恶性肿瘤细胞出现过度表达。为探讨Ｂｃｌ－２蛋白与光线性角化病（ＡＫ）的关系，作者用流式细胞免疫荧光技术，检测了２７例ＡＫ。结果显示：２７例ＡＫ及正常对照的Ｂｃｌ－２蛋白相对含量ＦＩ（ＦｌｕｏｒｅｓｃｅｎｃｅＩｎｄｅｘ）分别为１．１５０±０．１８８和０．９９６±０．０６５（Ｘ－±Ｓ），０．０５＜Ｐ＜０．０１。但其中８例不典型增生严重ＡＫ的ＦＩ为１．３３５±０．０１７６（Ｘ－±Ｓ），与正常对照相比Ｐ＜０．０２。提示该病的Ｂｃｌ－２蛋白有增高的倾向。The Bcl 2 protein is the expression of Bcl 2 gene, which has been proved to be a suppressor of apoptosis and was found in some malignant tumors. In order to explore the relation between Bcl 2 protein and actinic keratosis (AK), authors analysed 27 cases of AK with immunofluorescence staining technique and flow cytometry. The results showed that the fluorescence index (FI) representing the relative content of Bcl 2 protein in 27 cases of AK and normal control were 1.150±0.188,0.996±0.065 ( X- ± S ) respectively , 0.05< P <0.1. But the FI in 8 cases of severely dysplastic AK was 1.335±0.0176 ( X- ± S ) compared with control P <0.02. It suggested the existence of an tandency increased Bcl 2 protein in AK.

关 键 词：光线性 角化病 BCL-2蛋白 基因表达 

分 类 号：R739.502[医药卫生—肿瘤] R758.102[医药卫生—临床医学]