Melatonin　decreases　production　of　hydroxyl　radical　during　cerebral　ischemia-reperfusion  

作　　者：李学君[1] 张玲妹[1] 顾靖 张安中[1] 孙凤艳[1] 

机构地区：[1]上海医科大学神经生物学教研室

出　　处：《中国药理学报》1997年第5期394-396,共3页Acta Pharmacologica Sinica

摘　　要：目的：研究褪黑激素（ｍｅｌａｔｏｎｉｎ）对大鼠脑缺血再灌注中羟自由基生成的影响．方法：采用栓线法阻塞左侧ＭＣＡ３０ｍｉｎ再灌注模型．通过水杨酸捕获法与微透析技术结合来观察缺血再灌中羟自由基含量的变化．结果：ＤＨＢＡ水平在缺血１５ｍｉｎ后显著升高，持续到再灌注后３０ｍｉｎ仍维持较高水平．缺血前３０ｍｉｎ给予ｍｅｌａｔｏｎｉｎ（４ｍｇ·ｋｇ－１，ｓｃ）显著降低缺血１６－３０ｍｉｎ及再灌注１－３０ｍｉｎ时ＤＨＢＡ的含量．结论：Ｍｅｌａｔｏｎｉｎ对大鼠缺血再灌中羟自由基的产生有抑制作用．AIM: To study the effect of melatonin on hydroxyl radical ( ·OH ) contents during cerebral ischemia reperfusion in rats. METHODS: Ischemia was induced by occluding left lateral middle cerebral artery for 30 min following reperfusion. The salicylate trapping method coupled with ipsilateral striatal microdialysis for measurement of hydroxyl radicals generated during ischemia and reperfusion. RESULTS: The contents of dihydroxybenzoic acid (DHBA) were increased at 15 min after ischemia and remained high for 30 min after reperfusion. Melatonin (4 mg·kg -1 , sc, 30 min before ischemia) decreased the production of DHBA during ischemia for 16-30 min and reperfusion for 1-30 min . CONCLUSION: Melatonin inhibits the production of hydroxyl radicals in rat brain during ischemia and reperfusion.

关 键 词：褪黑激素 羟自由基 脑缺血 微透析 

分 类 号：R743.310.5[医药卫生—神经病学与精神病学]