雪旺细胞与聚乳酸/聚羟基乙酸丝相容性的研究  

Biocompatibility study of polylactic/giycolic acid and Schwann cell

作　　者：杜怀栋[1] 田宏斌[1] 周梁[1] 田洁[1] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院耳鼻咽喉科,上海200031

出　　处：《临床耳鼻咽喉头颈外科杂志》2008年第5期226-228,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：上海市科委资助研究项目(No:02J14026)

摘　　要：目的:研究雪旺细胞与聚乳酸/聚羟基乙酸(PLGA)丝的生物相容性。方法:应用乳鼠坐骨神经和臂从神经取材进行雪旺细胞培养,经阿糖胞苷抑制成纤维细胞及差数贴壁纯化,传代增殖至一定数量,将雪旺细胞与PLGA丝复合培养,经扫描电镜观察细胞的生长情况。结果:雪旺细胞可以在PLGA丝上贴壁生长,随着时间延长,细胞可分裂增殖。结论:雪旺细胞与PLGA丝生物相容性好,可进一步应用于神经修复。Objective.. To examine the biocompatibiLity of poLyLactic/giycohc acid （PLGA） anti Schwann CELLS. Method：Schwann cells were harvested from rat brachial and sciatic nerves. Schwann cells were cultured with PLGA, observed by phase-contrast microscopy and electron microscopy. Result： Schwann cells could attach and proliferate on the surface of the PLGA. Conclusion. The PLGA has good cellular biocompatibility. It can be used as biomaterial for tissue engineering.

关 键 词：雪旺细胞 聚乳酸/聚羟基乙酸丝 生物相容性 

分 类 号：R745.1[医药卫生—神经病学与精神病学]