一种适于介入性技术研究的肾动脉粥样硬化小型猪模型  

A new renal artery atherosclerosis mini pig model suitable for interventional studies

作　　者：张鹏飞[1] 李继福[1] 张梅[1] 丁士芳[1] 徐新生[1] 李晓楠[1] 张运[1] 刘春喜[2] 梁向明[3] 张彦恩[4] 崔先泉[5] 

机构地区：[1]山东大学齐鲁医院心内科,济南 250012 [2]卫生部和教育部心血管重构与功能研究重点实验室 [3]山东大学齐鲁医院泌尿内科,济南 250012 [4]山东大学齐鲁医院心外科,济南 250012 [5]山东大学齐鲁医院血液净化中心,济南 250012

出　　处：《中华肾脏病杂志》2008年第3期196-201,共6页Chinese Journal of Nephrology

基　　金：：国家自然科学基金（60402023）;山东省自然科学基金（Q2006C11）

摘　　要：目的建立一种适于介入性技术研究的肾动脉粥样硬化小型猪动物模型。方法纯种系中国实验用小型猪（CEMP）8只，饲以高脂饮食13周后，以过大球囊拉伤单侧肾动脉，并继续高脂饲料喂养至40周。分别测定第1、14、40周的血脂和血肌酐水平，并于第14和40周行血管内超声检查。40周处死动物后，采集标本行苏木素一伊红、Masson三色和油红O染色及抗巨噬细胞免疫组织化学染色。结果第40周CEMP血脂和血肌酐水平较第1周明显升高，损伤侧。肾脏出现灶性缺血性病理改变。CEMP。肾动脉适于血管造影和介入性血管内超声检查，后者提供了清晰的血管截面病变信息，损伤侧。肾动脉内膜．中层厚度与未损伤侧比较，差异有统计学意义[（0．89±0．03）mm比（0．30±0．02）mm，P〈0．05]。病理检查证实肾动脉病变符合粥样硬化特征，形成的斑块中，纤维性占85％，纤维脂质性占15％。结论成功建立了模拟动脉粥样硬化性。肾血管病变发生发展和适于介入性技术研究的。肾动脉粥样硬化小型猪动物模型。血管内超声技术对于动脉粥样硬化性肾血管病变的评价具有潜在的临床应用前景。Objective To establish a mini pig model suitable for interventional studies in vivo. Methods The endothelia of unilateral renal arteries in 8 purebred Chinese experimental mini pigs （CEMP） was denuded by inflated balloons after the animals were fed with high cholesterol diet for 13 weeks. The CEMP were fed with high cholesterol diet continuously till the 40th week. The levels of blood lipid panel and creatinine were tested at week 1, 14 and 40. Bilateral renal arteries were examined with intravascular ultrasonography at week 14 and 40. The vessel samples were collected at week 40 and stained with haematoxylin-eosin, Masson trichrome technique, oil O and anti-macrophage immunohistological technique. Results Significant differences of blood lipid panel and creatinine were found between week 1 and week 40. Focal ischemic renal injury could be observed pathologically. Renal arteries of CEMP were suitable for interventional procedure such as angiography and intravascular ultrasonography. Cross-sectional information of vessles could be provided clearly by intraveascular ultrasonography and the intimamedian thickness of injured renal arteries was much thicker thn that of non-injured ones [0.89±0.03）mm vs （0.30±0.02）mm,P〈0.05] as evidenced by this diagnostic technique.Pathological findings demonstrated the atherosclerotic proliles of the injured renal arteries.Fibrous and fibro-fatty with renal arterial atherosclerosis mimicking the progression of atherosclerotic renovascular disease,which is suitable for interventional procedure is established successfully.Intravascular ultasonography may have potentia clinical prospect on the evaluation of atherosclerotic renovas cular disease.

关 键 词：肾动脉 动脉粥样硬化 动物 实验 介入性技术 血管内超声 小型猪 

分 类 号：R-332[医药卫生]