新型Barrett食管、食管腺癌动物模型的建立及环氧合酶-2对其发生的影响  

Animal model of Barrett′s esophagus and esophageal adenocarcinoma and expresssion of cyclooxygenase-2

作　　者：张冬梅[1] 王雯[2] 王蓉[1] 李达周[2] 刘建强[2] 

机构地区：[1]南京军区福州总医院老年病二科,350025 [2]南京军区福州总医院消化内科,350025

出　　处：《实用医学杂志》2008年第6期912-914,共3页The Journal of Practical Medicine

基　　金：福建省青年创新人才项目课题(编号:2002J059)

摘　　要：目的:建立新型安全的Barrett食管(BE)及食管腺癌(EA)动物模型,并探讨环氧合酶-2(COX-2)表达在BE、EA发生发展中的作用。方法:150只SD大鼠分为对照组、铁剂组、反流组、反流铁剂组,后两组行手术造成十二指肠胃食管反流,铁剂组、反流铁剂组大鼠腹腔注射右旋糖酐铁(5mg/kg,每周2次,共22周)。术后8周每组各处死5只,24周后全部处死,取得食管标本进行组织形态学研究观察大鼠食管BE及EA的发生情况,应用SP免疫组化染色检测COX-2的表达。结果:对照组与铁剂组未发生BE和EA,反流铁剂组BE、异型增生及EA的发生率高于反流组(P<0.05)。反流铁剂组COX-2表达阳性率及阳性程度高于其他组(P<0.05)。COX-2在肿瘤中表达最高,异型增生中次之,正常黏膜表达最低。结论:应用手术致食管反流并注射铁剂的方法制作大鼠BE及EA模型成功率高,安全性好。COX-2表达的增加可能与BE及EA的发生发展有关。Objective To establish the rat model of Barrett＇s esophagus（BE） and esophageal adenocarcinoma （EA） and to investigate the role of cyclooxygenase-2 （COX-2） in the development of BE and EA. Methods One hundred and fifty Sprague-Dawley rats were randomized into 4 groups, including control （no reflux） group, iron（Fe） group, reflux group and reflux adding iron group. Esophagoduodenostomy was made in the last two groups and Iron Dextran were injected into abdominal cavity of the rats in Fe group and reflux adding iron group （50 mg/kg, twice per week for 22 weeks）. Five rats of each group were sacrificed at 8 week after operation and others at 24 week, and esophageal samples were taken. All esophagi were assessed for presence of cancer, BE, and dysplasia by gross and microscopy observation. COX-2 expression was examined in all groups by immunohistochemical staining of S-P. Results At 24 week, the incidence of BE（57.1% vs 28.9%, P 〈 0.05）, dysplasia（42.9% vs 34.2%, P 〈 0.05） and EA （20.0% vs 2.6%, P 〈 0.05） were increased significantly in reflux adding iron group compared with refulx group, and both of them were significantly more than those in the other two groups （P 〈 0.05）. The expression of COX-2 in reflux adding iron group and reflux group was significantly greater than those in control group and ire group （P 〈 0.05）, and then was the strongest in EA. Conclusions Iron dextran can increase the injury of gastroduodenalesophageal reflux on esophageal mucosa and induce the developement of BE and EA. This model is safe and successful. Increased expression of COX-2 may be associated with the development of BE and EA.

关 键 词：BARRETT食管 腺癌 食管 胃食管反流 环氧水解酶类 

分 类 号：R-332[医药卫生]