P16^(INK4A)和人乳头瘤病毒检测在液基细胞学筛查宫颈病变中的应用  

Value of P16^(INK4A) expression and human papillomavirus test determined by liquid-based cytology of cervical specimens in investigating cervical lesions

作　　者：任力[1] 狄文[1] 何以丰[2] 杨卉[1] 

机构地区：[1]上海交通大学医学院附属仁济医院妇产科,上海200001 [2]复旦大学附属妇产科医院,上海200011

出　　处：《诊断学理论与实践》2008年第1期80-82,共3页Journal of Diagnostics Concepts & Practice

摘　　要：目的:探讨P16INK4A和高危型人乳头瘤病毒(HR-HPV)在宫颈癌早期及癌前病变中的表达及液基细胞学标本在P16INK4A检测中的应用。方法:选取8993名宫颈炎患者进行液基薄层细胞学检查,其中有64例呈不良反应,应用免疫组织化学S-P方法检测其P16INK4A表达,同时采用实时定量PCR法检测HPV表达。结果:P16INK4A在低度鳞状上皮内病变(LSIL)中的阳性率为28.6%,高度鳞状上皮内病变(HSIL)中的阳性率为81.8%,鳞状细胞癌(SCC)中的阳性率为100.0%。随着宫颈病变级别的上升,P16INK4A及HR-HPV阳性率均相应增高。结论:P16INK4A的表达与宫颈癌的发生、发展相关,并与宫颈病变分级及HPV感染高度相关,是筛查液基细胞学组织标本宫颈病变的一个有用指标。Objective To study the value of expression of P16^INK4A and high-risk human papillomavirus （HR-HPV） in investigating cervical cancer and pre-malignant lesion and to confirm the usefulness of liquid-based cervical specimens for P16^INK4A staining. Methods liquid-based cytologic examinations underwent in specimens of 8993 patients with cervicitis. Of the speciments, 64 specimens with abnomal reactivity were studied for antibody P16^INK4A by immunohistochemical method. Moreover, the state of the HPV infection was examined by real-time polymerase chain reaction（PCR）. Results The P16^INK4A-positive values in low-grade squamous intraepithelial lesion （LSIL） were 28.6%, high-grade squamous intraepi-thelial lesion （HSIL） were 81.8%, and squamous cell carcinoma （SCC） were 100.0%, respectively. Positive ratio of P16^INK4A and HR-HPV increased with the cervical lesions’ grade. Conclusions The presence of P16^INK4A immunoreactivity is a good predictor of dysplasia in liquid-based cervical specimens.

关 键 词：P16^INK4A 人乳头瘤病毒 液基细胞学 宫颈病变 

分 类 号：R737.33[医药卫生—肿瘤]