乳酸菌对肠粘膜通透性及中小分子尿毒素清除的影响  

The influence of lactobacillus on the permeability of intestinal mucosa and the clearance of middle and low molecular uremic solutes in rats

作　　者：贺丽娟[1] 蒋哲峰[2] 蒋云生[1] 熊文[1] 焦闻文[1] 

机构地区：[1]中南大学湘雅二医院肾病科 [2]中南大学湘雅二医院检验科,长沙410011

出　　处：《中国血液净化》2008年第2期78-80,共3页Chinese Journal of Blood Purification

基　　金：国家自然科学基金资助项目(30670974)

摘　　要：目的研究乳酸菌对慢性肾衰竭大鼠的肠粘膜通透性及中小分子尿毒素清除的影响。方法40只SD大鼠分为正常对照组(10只)行假手术,30只作5/6肾切除后分为肾衰竭病理对照组、双歧杆菌治疗组和乳酸杆菌治疗组。喂养1周后处死动物留取血粪标本。用紫外线吸收法测定血/粪中分子物质(MMS)。尿素(UN)和肌酐(Cr);用酶偶联紫外分光度法检测血D-乳酸。结果病理对照组血、粪MMS和UN、Cr均升高(P<0.01);血浆D-乳酸含量增高(P<0.01)。双歧杆菌治疗组和乳酸杆菌治疗组较病理对照组的血浆D-乳酸含量降低(P<0.01);BUN、SCr均降低(P<0.01);而粪UN、Cr均增加(P<0.01)。结论双歧杆菌和乳酸杆菌均可维持慢性肾衰竭大鼠肠粘膜正常通透性,加速肠道清除MMS,分解肠道内尿素和肌酐降低其血中的浓度。Objective To investigate the effect of intestinal lactobacillus on the maintenance of intestinal mucosa permeability and the clearance of middle and low molecular uremic solutes in rats. Methods Forty Sprague-Dawley rats were divided into 4 groups： normal control group （10 rats）, with sham operation; pathological control group, 5/6 nephrotomized rats without treatment; lactobacillus treated group, 5/6 nephrotomized rats treated with lactobacilli; bifidobacterium treated group, 5/6 nephrotomized rats treated with bifidobacteria. Animal were sacrificed and blood and stool samples were taken after feeding the bacteria for one week. Middle molecular substance （MMS）, urea and creatinine （Cr） in blood and stool were determined. Plasma D-lactic acid was measured by enzyme-coupled ultraviolet spectrophotometry. Results MMS, urea and Cr in blood and stool and D-lactic acid in plasma were increased in pathological control group （P〈0.01）. In lactobacillus and bifidobacterium treated groups, however, plasma D-lactic acid, blood urea nitrogen and serum Cr decreased remarkably （P 〈 0.01）, and urea nitrogen and Cr in stool increased （P 〈 0.01）. Conclusions Bifidobacteria and lactobacilli can preserve the normal permeability of intestinal mucosa, promote the clearance of MMS, urea and Cr through intestinal tract, degrade intestinal urea and Cr, and thereby reduce blood urea and Cr.

关 键 词：5/6肾切除 肠粘膜通透性 乳酸菌 中分子物质 

分 类 号：R692.5[医药卫生—泌尿科学]