细胞角质素CK18和CK19在肝细胞癌组织中的表达  

Expressions of cytokeratin 18 and cytokeratin 19 in hepatocellular carcinoma tissues

作　　者：李蔚[1] 周慧聪[2] 李继昌[1] 段芳龄[3] 

机构地区：[1]郑州大学第一附属医院消化内科,河南省郑州市450052 [2]河南职工医学院内科教研室,河南省郑州市450000 [3]郑州大学第二附属医院消化内科,河南省郑州市45000

出　　处：《世界华人消化杂志》2008年第7期721-725,共5页World Chinese Journal of Digestology

摘　　要：目的:观察和探讨细胞角质素CK18和CK19在不同肝组织中的表达及其意义.方法:采用免疫组化SP法观察正常肝组织8例,肝硬化组织27例和肝细胞癌(hepatocellular carcinoma,HCC)组织43例中CK18,CK19的表达.结果:肝CK18和CK19在肝硬化组与正常肝组织的表达率均无明显差异,但两者在肝细胞癌组与肝硬化组之间差异有显著性(65.1%vs29.6%,69.8%vs25.9%,P<0.01).在20/27例肝硬化组织和35/43例HCC组织中可见到CK18,CK19标记的卵圆细胞,且在两种组织中卵圆细胞的数量有显著性差异(CK18:6.57±1.69vs10.70±2.31;CK19:5.37±1.17vs10.45±2.15,P<0.01).结论:CK18和CK19参与了肝硬化到HCC的癌变过程;CK18和CK19在卵圆细胞中的强阳性表达,支持HCC的卵圆细胞起源学说。AIM： To observe the expressions of cytoketatin18 （CK18） and cytoketatin19 （CK19） in tissues of hepatocellular carcinoma, liver cirrhosis, and normal liver. METHODS： Immunohistochemical streptavidin-peroxidase （SP） method was adopted to examine the expression of CK18 and CK19 in tissue samples of normal liver （n = 8）, liver cirrhosis （n = 27）, and hepatocellular carcinoma （n = 43）. RESULTS： The positive rates of CK18 expression in hepatic cirrhosis and normal liver tissues had no significant differences. However, CK18 expression was significantly different betweenhepatocellular carcinoma and liver cirrhosis （65.1% vs 29.6%, P 〈 0.01）. The positive rates of CK19 expression in cirrhosis of liver and normal liver had no significant differences. But the expression of CK19 was markedly higher in hepatocellular carcinoma than that in hepatic cirrhosis （69.8% vs 25.9%, P 〈 0.01）. Oval cells with strongly positive staining could be seen in the portal area of cirrhosis cases （20/27） and in the brink of carcinoma cases （35/43）, and there were significant differences （CK18：6.57 ± 1.69 vs 10.70 ± 2.31; CK19：5.37 ± 1.17 vs 10.45 ± 2.15, P 〈 0.01） in the numbers between cirrhosis of liver and hepatocellular carcinoma. CONCLUSION： CK18 and CK19 are involved in hepatocarcinogenesis. Oval cells are strongly positive for CK18 and CK19 in cirrhosis of liver and hepatocellular carcinoma. Oval cells are associated with regeneration of liver, and are probably original cells of hepatocellular carcinoma.

关 键 词：角蛋白 肝癌 卵圆细胞 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤] R734.2[医药卫生—临床医学]