胃癌中高表达ATP/GTP结合蛋白1基因的筛选及验证  

Screening and identification of ATP/GTP binding protein 1 gene expressed highly in gastric cancer

作　　者：张宇川[1] 张尤历[1] 王文兵[2] 高广[2] 陆芬英[1] 

机构地区：[1]江苏大学附属医院消化科,江苏省镇江市212001 [2]江苏大学生命科学院,江苏省镇江市212001

出　　处：《世界华人消化杂志》2008年第7期780-783,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨胃癌中差异表达的基因在胃癌发生和发展的分子机制中的作用.方法:提取胃癌组织样本的总RNA,通过荧光差异显示技术(DD-PCR)获得胃癌样品中差异的片段,对这些片段进行克隆和测序.通过在GenBank中同源性检索,查找与差异片段相对应的同源基因.利用半定量PCR及定量PCR方法验证该基因表达的差异性.结果:通过DD-PCR得到差异片段中的一个片段对应与人ATP/GTP结合蛋白1基因(A/GTPBP1).半定量PCR及荧光定量PCR技术检测结果表明,A/GTPBP1基因在胃癌组织中的表达量高于其对应的癌旁组织.该基因的读码框长3561bp,编码1186个氨基酸,分子质量为84.4kDa,蛋白质相似性分析表明该蛋白为G蛋白家族成员.结论:A/GTPBP1在胃癌组织中异常高表达,可能在胃癌发生过程中起调节作用。AIM： To investigate the molecular mechanism of gastric carcinogenesis, screen and validate the genes expressed differently in gastric cancer. METHODS： Messenger RNA differential display polymerase chain reaction （DD-PCR） was employed to search differently expressed fragments, some of which were cloned and sequenced. By homologous analysis in GenBank, the corresponding homologous genes of those fragments were found. The corresponding genes of those differently expressed fragments were validated by real-time semi-quantitative and quantitative PCR. RESULTS： By DD-PCR, one expressed sequence tag （EST） was identified to be ATP/GTP binding protein 1 （A/GTPBF1） gene. The result of realtime semi-quantitative and quantitative PCRshowed that the expression level of A/GTPBP1 gene in gastric cancer tissues was significantly higher than that in normal tissues. The open reading frame （ORF） of A/GTPBP1 gene, which encodes 1186 amino acids, was 3561-base pair long. The molecular weight was about 84.4 kDa. Protein sequence similarity analysis showed that A/GTPBP1 was a member of the G protein family. CONCLUSION： The over-expression of AGTPBP1 gene in gastric cancer may play an important role in gastric carcinogenesis.

关 键 词：荧光差异显示 定量聚合酶链反应 胃癌 ATP/GTP结合蛋白1基因 

分 类 号：R735.2[医药卫生—肿瘤]