鸭MHCⅡα融合蛋白表达条件的优化  

Optimization of Expression Conditions of Duck MHCⅡα

作　　者：张舒婕[1] 邓干臻[1] 龚炎长[1] 俸艳萍[1] 杨庆磊[1] 高俊伟[1] 彭秀丽[1] 

机构地区：[1]华中农业大学动物遗传育种与繁殖教育部重点实验室,武汉430070

出　　处：《华中农业大学学报》2008年第1期71-74,共4页Journal of Huazhong Agricultural University

基　　金：湖北省"十一五"重大攻关项目(2006AA202A03)资助

摘　　要：构建了鸭MHCⅡα的融合表达载体PET-28a,对融合蛋白在大肠杆菌中表达量的影响因素进行了研究。SDS-PAGE分析表明,诱导温度22℃,时间3 h,IPTG浓度0.05 mmol/L,OD600 nm值0.45,通气量90%和pH 7.2时MHCⅡα融合蛋白的表达量最高,占菌体总蛋白的23.4%,比优化前提高了16.7%。蛋白经纯化后纯度可达90%。Western blot分析表明,该蛋白可被鼠抗鸭MHCⅡα阳性血清识别,具有较好的免疫学活性。This paper studied the influencing factor of duck MHCⅡα induce expression level of fusion protein in E.coli by SDS-PAGE.The result showed at the condition of 22 ℃,it induced 3 h,0.05 mmol/L IPTG,initiatal OD600 nm value 0.45,ventilatory capacity 90% and pH 7.2,the fusion protein expressed efficiently.The fusion protein was obtained in E.coli BL21 more than 23.4%,it has increased by 16.7%.The protein was purified and the purity reached 90%,which showed well immunological activity by Western-blot.

关 键 词：鸭 MHCⅡα 融合蛋白 表达条件 

分 类 号：Q782[生物学—分子生物学]