粘类小麦育性恢复基因的遗传分析及SSR分子标记  

Genetis analysis and SSR markers on fertility restorer gene of male sterility of wheat with Aegilops kotschyi cytoplasm

作　　者：李红霞[1] 张改生[1] 张龙雨[1] 牛娜[1] 

机构地区：[1]西北农林科技大学陕西省作物杂种优势研究与利用重点实验室,小麦育种教育部工程研究中心,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第4期65-68,74,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(301705760);国家“863”重大专项(2002AA207004);陕西省13115重大专项(2007ZDKG-02);国家杨凌农业生物技术育种中心专项(99-1A)

摘　　要：【目的】进一步探讨粘类小麦育性恢复基因的遗传机理。【方法】选取具有高恢复力的恢复系亲本材料rk5451为父本与ms(Kots)-90-110杂交,F1代再与保持系90-110回交,以ms(Kots)-90-110/rk5451//90-110的BC1F1代分离群体为研究对象,分别用定位于1B短臂染色体上的18对SSR引物和6B染色体上的47对SSR引物对粘类小麦细胞质雄性不育育性恢复基因进行分子标记。【结果】初步筛选出Wms273和Wmc406 2对揭示育性恢复基因多态性的引物,用132株BC1F1回交群体对这2对引物进行进一步检测,得出Wmc406与粘类小麦细胞质雄性不育系1BS上的恢复基因连锁,遗传距离约为7.6 cM。【结论】粘类小麦细胞质雄性不育系的育性是由1对主效恢复基因和多对微效基因共同控制的,标记Wmc406可直接用于分子标记辅助选择。【Objective】 In order to probe into the fertility characteristics of male sterile lines of Nian type,genetic index of fertility restorer gene for male sterile line of Nian type was analyzed.【Method】 The sterile line ms（Kots）-90-110 was used as female to cross with rk5451.SSR markers which were located on the chromosome 1BS and 6B were employed to tag the major restorer gene in BC1F1 of ms（Kots）-90-110/rk5451//90-110.【Rusult】 Two primer pairs（Wms273 and Wmc406） displayed polymorphism of the restorer gene.By analyzing the polymorphic markers in these segregating populations,the microsatellite locus Wmc406 located on chromosome 1BS was found to be linked to the restorer gene with the estimated genetic distance of 7.6 cM.【Conclusion】 Genetis analysis probe that fertility restorer gene of wheat male sterility of Nian type was influenced by major fertility restorter gene and some minor enhance gene.Wmc406 can be used in MAS directly.

关 键 词：粘类小麦雄性不育系 恢复基因 SSR分子标记 遗传学分析 

分 类 号：S512.103[农业科学—作物学]