神经肽Y对大鼠心肌细胞胞浆钙及钙瞬变的影响  

Effect of neuropeptide Y on calcium in plasma and Ca^(2+) transient of cardiomyocytes in rats

作　　者：张弋[1] 苏涛[2] 刘世明[3] 董颀[4] 陈敏生[3] 

机构地区：[1]广州医学院第二附属医院急诊科,广东广州510260 [2]广州医学院第二附属医院神经科学研究所,广东广州510260 [3]广州医学院第二附属医院心脏内科,广东广州510260 [4]广州医学院生理教研室,广东广州510182

出　　处：《中国现代医学杂志》2008年第6期719-721,共3页China Journal of Modern Medicine

基　　金：国家自然科学基金资助项目(No.30440053;No.30570759);广东省自然科学基金资助项目(No.04004529)

摘　　要：目的观察神经肽Y(neuropeptide Y,NPY)对静息状态下心肌细胞胞浆钙含量及兴奋-收缩耦联过程中心肌细胞钙瞬变的影响。方法用NPY(100nmol/L)刺激Sprague-Dawley乳鼠心肌细胞24h,用荧光染料Fluo4-AM负载胞浆钙,记录静息状态下心肌细胞胞浆钙浓度,并用场刺激诱发胞浆钙瞬变。所有钙影像均由LeicaSP2激光共聚焦显微镜记录。结果NPY组心肌细胞胞浆钙含量明显高于对照组(P<0.05);场刺激诱导下NPY组心肌细胞胞浆钙瞬变幅度明显高于对照组(P<0.01),NPY组钙瞬变恢复时间明显缩短(P<0.01),钙瞬变上升时间则无显著差别。结论长时间的NPY刺激可显著影响兴奋-收缩耦联过程中钙的动态活动,并导致胞浆内钙含量增加。[Objective] To investigate the effect of neuropeptide Y （NPY） on calcium （Ca^2＋）in plasma and Ca^2＋ transients of rat cardiomyoeytes. [Method] Cardiomyocytes of neonatal Sprague-Dawley rats were incubated with NPY （100 nmol/L） for 24 h. Fluorescent assay with Fluo 4-AM was used to detect Ca^2＋ in plasma and Ca^2＋transients of cardiomyocytes through laser scanning confocal microscope under the field. [Result] 24 hours after incubation, the Ca^2＋ in plasma cardiothyocyte in NPY group was significantly elevated compared with control group （P 〈0.05）; Under the field stimulation, the evoked Ca^2＋ transient was of higher amplitude and of faster decay in the presence of NPY（P 〈0.01）. The time-to-peak-Ca^2＋ was of no significant change. [Conclusion] NPY stimulation can significantly impact dynamic activity of calcium in excitation-contraction coupling process and cause the enhancement of Ca^2＋ in plasma.

关 键 词：神经肽Y 心肌细胞 胞内游离钙 钙瞬变 

分 类 号：R-332[医药卫生]