重复加热对肝癌HepG2细胞谷胱苷肽-S-转移酶π表达的影响  

The expression of GSTπ in the HepG2 cell after multiple thermotherapy

作　　者：秦春宏[1,2] 李永国[2] 吴江[1] 何苦寒[1] 申昕[1] 费书柯[1] 

机构地区：[1]南华大学附属第二医院普外科,湖南衡阳421001 [2]中南大学湘雅二医院肝胆外科

出　　处：《中国医师杂志》2008年第3期297-299,共3页Journal of Chinese Physician

摘　　要：目的探讨多次加热后对残存的肝癌HepG2细胞的谷胱苷肽-S-转移酶竹（GSTπ）基因／蛋白表达的影响。方法HepG2细胞43℃加热，每次80min，2次／d，10个循环后，MTT法分析其对阿霉素的敏感性，免疫组化法检测细胞GSTπ蛋白的表达。RT-PCR法检测细胞GSTπmRNA的表达。结果热处理后的HepG2细胞对阿霉素的化疗耐受性升高，GSTπ基因／蛋白的表达增强。结论热处理后HepG2细胞对化疗药物的耐受性可能与其GSTπ基因／蛋白的表达增强有关。Objective To investigate the expression change of glutathione S-transferase π（GSTπ） protein and mRNA in the HepG2 cell after multiple thermotherapy. Methods HepG2 cells were treaded by ten repeated cycles of exposure at 43 degree C for 80 minutes twice a day, the sensibility of HepG2 cell to Adriarnycin was analyzed by MTT assay, and the expression of GST=protein and mRNA were detected by immunohistoehemical method and RT-PCR. Results The drug resistance to Adriamycin was gained by HepG2 cells after multiple thermotherapy, and the expression of GSTπ protein and mRNA was strengthened. Conclusions The resistance of heated HepG2 cells to chemotherapeutics was concerned with overexpression of GSTπ protein and mRNA.

关 键 词：高温 诱发 肝肿瘤 谷胱甘肽转移酶 

分 类 号：R735.7[医药卫生—肿瘤] R735.2[医药卫生—临床医学]