CK-19mRNA、CEAmRNA检测在肺癌围术期的应用及其意义  

The sigmificance of CK-19 mRNA and CEA mRNA in the circulating vein blood of non-small cell lung cancer

作　　者：王保祥[1] 张位星[1] 陈胜喜[1] 罗万俊[1] 过江[1] 艾正华[1] 

机构地区：[1]中南大学湘雅医院心胸外科,湖南长沙410008

出　　处：《中国医师杂志》2008年第3期330-332,共3页Journal of Chinese Physician

摘　　要：目的探讨肺血管处理顺序的选择对癌细胞入血的影响。方法收集56例手术治疗的非小细胞肺癌患者，术前随机分为2组，即：先结扎肺静脉组和先结扎肺动脉组；术前1d和术后第7天分别采集患者的外周静脉血，用巢氏PCR检测外周静脉血中CK-19mRNA、CEAmRNA的表达。结果术后患者外周静脉血中CK-19mRNA、CEAmRNA的表达低于术前，先结扎肺静脉组的患者术后外周血中CK-19mRNA、CEAmRNA表达的阳性率低于先结扎肺动脉组。结论手术操作影响癌细胞的入血。先结扎肺静脉可减少癌细胞入血。Objective To detect CK19 mRNA and CEA mRNA in blood of the peripheral vein and to investigate the effect of liga-ting pulmonary vein firstly or ligating pulmonary artery firstly during surgical operation on haematogenous dissemination of malignant cells. Methods Fifty six non - small cell lung cancer patients were collected and random assigned to two groups before operation （ ligatlng pulmo-nary vein firstly or ligating pulmonary artery firstly）. The patients were accepted radical operation and their operations were put in practice by doctors of one team. Vein blood was collected one day before operation and one week after operation. CK19 mRNA and CEA mRNA in blood were detected by nested reverse transcriptase-polymerase chain reaction. Result The positive rate of expression of CK19 mRNA and CEA mRNA after operation is lower than that before operation. The positive rate of expression of CK19 mRNA and CEA mRNA in ligating pulmo- nary vein firstly after operation is lower than that in the other group, Conclusions Surgical operation have effects on the dissemination of malignant cell and ligating pulmonary vein firstly during operation can reduce the dissemination of malignant cell.

关 键 词：癌 非小细胞肺 角蛋白 癌胚抗原 手术期间 

分 类 号：R734.2[医药卫生—肿瘤]