油茶FAD2基因全长cDNA的克隆和序列分析  

Cloning of Full-Length cDNA of FAD2 Gene from Camellia oleifera

作　　者：谭晓风[1] 陈鸿鹏[1] 张党权[1] 曾艳玲[1] 李魏[1] 蒋瑶[1] 谢禄山[1] 胡孝义[1] 胡芳名[1] 

机构地区：[1]中南林业科技大学经济林育种与栽培国家林业局重点实验室,长沙410004

出　　处：《林业科学》2008年第3期70-75,共6页Scientia Silvae Sinicae

基　　金：国家"十一五"科技支撑项目(2006BAD18B0204;2006BAD01A1706);国家自然科学基金(30371184);湖南省自然科学基金(07JJ3070);湖南省科学技术厅科技计划项目(06FJ4111)

摘　　要：FAD2基因控制油酸转化为亚油酸,是油茶油脂合成代谢的关键酶基因。在构建的油茶EST文库基础上,采用5′RACE和交错延伸PCR技术获得了油茶FAD2基因的全长cDNA克隆。该基因序列长1682bp,开放阅读框为1149bp,编码382个氨基酸,并且具有FAD2特有的保守序列和相关特征。经过比对分析,发现油茶的FAD2基因与其他植物的FAD2基因具有较高的相似性。Camellia Oleifera is an important oil-producing woody species in China, and the oil, called as tea-oil, can be used as a high-quality edible oil, cosmetic material and biomass energy with the abundant poly unsaturated fatty acids such as oleic acid and linoleic acid. FAD2 gene is a key factor in controlling lipid biosynthesis, and controls the formation of linoleic acid from oleic acid in seeds of C. oleifera. Cloning of the FAD2 gene would facilitate us to reveal the lipid synthesis rule in seeds of C. oleifera and have important impact on theory and application. Full-length cDNA clone of FAD2 gene was for the first time obtained by using methods of 5＇RACE and overlap extension PCR based on our constructed EST library of C. oleifera. It was 1 682 bp in length and contained a 1 149 bp ORF （open reading frame） encoding a peptide of 382 amino acids, which had the typical conserved domains of FAD2 and showed high homology with those of other plant species.

关 键 词：油茶 EST文库 FAD2基因 RACE 交错延伸PCR 基因克隆 

分 类 号：S718.46[农业科学—林学] Q943.2[生物学—植物学]