姜黄素对gp120所致大鼠学习记忆障碍的作用及机制研究  

Effect and mechanism of curcumin on learning and memory dysfunction induced by gp120 in rats

作　　者：董军[1] 陆大祥[1] 潘锐[2] 唐红梅[1] 

机构地区：[1]暨南大学医学院病理生理教研室 [2]暨南大学附属第一医院骨科,广东广州510632

出　　处：《细胞与分子免疫学杂志》2008年第4期328-331,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：广东省自然科学基金资助项目(04010443,06105246);广东省医学科研课题资助项目(A2004327,A2006334);广州市科技计划项目资助(2007J1-C0041);重点实验室开发基金资助项目(51206011,51207029)

摘　　要：目的:探讨姜黄素改善HIV-1包膜糖蛋白gp120所致大鼠学习记忆障碍的作用及机制。方法:用gp120侧脑室灌注制备拟艾滋痴呆症动物模型,并用水迷宫实验观察侧脑室灌注gp120造成的大鼠认知功能的障碍。SD大鼠随机分为6组:对照组、假手术组、模型组、姜黄素低、中和高剂量治疗组。除对照组、假手术组外其余4组侧脑室缓慢注射5μL的gp120,连续3d。第4天开始,姜黄素低、中、高剂量治疗组分别给予50mg/(kg·d)、100mg/(kg·d)、200mg/(kg·d)的姜黄素灌胃,对照组、假手术组和模型组大鼠用双蒸水灌胃,连续灌胃14d。然后各组大鼠进行水迷宫测试,并分组进行NMDA2B受体免疫组化染色。结果:50ng/d的gp120侧脑室灌注3d,可制备拟艾滋痴呆症动物模型。Morris水迷宫可见模型组大鼠逃避潜伏期与对照组相比明显延长(P<0.05),姜黄素低、中、高剂量治疗组大鼠的逃避潜伏期与模型组相比缩短,其中姜黄素低剂量组效果更好(P<0.05)。免疫组化结果显示模型组大鼠海马内N-甲基天冬氨酸受体(NMDA2B)的表达与对照组相比有所降低(P<0.01),姜黄素各剂量治疗组NMDA2B受体的表达与模型组相比有所上调。结论:gp120侧脑室灌注可制备拟艾滋痴呆症动物模型,姜黄素具有改善侧脑室灌注gp120所致大鼠学习记忆障碍的作用,其机制可能与对抗NMDA2B受体表达下调有关。AIM： To explor the effect and mechanisms of curcumin on learning and memory dysfunction induced by HIV-1 enveloped protein gpl20. METHODS： The SD rats were treated with gpl20 by intracerebroventricular （ICV） infusion imiating the HIV-1 associated dementia （HAD） animal model. Subsequently, We applied the water maze test to evaluate the effect of gpl20 on the learning and memory dysfunction in rats. The SD rats were divided into six groups： control group, sham group, model group, low dose curcumin group, middle dose curcumin group and high dose curcumin group. Except control and sham group, the others four groups received slowly 5 μL/d gpl20 which dissolved in artificial cerebrospinal fluid （ACSF） for 3 days. Since the fourth day, the rats of low, middle, high dose curcumin groups were treated with 50 mg/（kg ·d）, 100 mg/（kg · d）, 200 mg/（kg · d） curcumin, respectively. The others groups were treated with redistilled water. The treatment lasted for 14 days. Subsequently, the water maze test and NMDA28R immunohistochemical staining were applied to evaluate the effect of curcumin on the rats. RESULTS： The rats were treated with gpl20.50 ng/d by ICV infusion for 3 days can imiate the HAD animal model. The Morris water maze（MWM） test showed that the rats in model group had longer escape latencies compared with those in control group （P〈0.05） and that rats in low, middle, high dose cumumin groups had shorter escape latencies compared with those in model group （P 〈 0.05 ）, and low dose curcumin group was better than the other two groups （ P 〈 0.05）. Immunohistochemical staining showed that the expressions of NMDA2BR in model group decreased compared with the control groups （P 〈 0.01 ）, while the expressions of NMDA2B R in low, middle and high dose curcumin groups increased compared with the model groups. CONCLUSION： The SD rats were treated with gpl20 by ICV infusion imiating the HAD animal model. The curcumin can improve the learning and memory dysfunction

关 键 词：艾滋痴呆症 GP120 姜黄素 N-甲基天冬氨酸受体 

分 类 号：R285.5[医药卫生—中药学]