巯基乙酸对小鼠成熟卵母细胞皮质颗粒分布和MAPK活性的影响  

Effects of Thioglycolic Acid on Cortical Granule Redistribution and MAPK Activation in Mouse Oocytes

作　　者：侯绍英[1] 张岭[1] 吴坤[1] 夏蕾[2] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,黑龙江哈尔滨150081 [2]哈尔滨医科大学附属第一医院生殖医学中心,黑龙江哈尔滨150001

出　　处：《癌变．畸变．突变》2008年第2期107-110,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(30400356);黑龙江省卫生厅项目(2006-032)

摘　　要：背景与目的:探讨巯基乙酸(thioglycolic acid,TGA)对小鼠卵母细胞胞浆成熟和相关生化指标的影响。材料与方法:以不同浓度TGA(0.2、1.0、2.5mmol/L)体外培养小鼠卵母细胞,并设M16培养液对照组,培养16h后在体视显微镜下,观测卵母细胞生发泡破裂和第一极体排出情况,用免疫荧光染色方法对皮质颗粒(cortical granules,CGs)进行标记,采用western blot方法对p44/42MAPK进行检测。结果:各组生发泡破裂率均达到90%左右,各组间差异无统计学意义(P>0.05)。随着TGA剂量的增加,第一极体排出率下降,各剂量组和对照组相比差异均有统计学意义(P<0.05)。各剂量组均可观察到CGs在细胞膜下的线状排列,但随着TGA剂量的增加,质膜下CGs的密度降低,胞浆中CGs的密度有增加的趋势。对照组卵母细胞中有非常明显的无皮质颗粒区(cortical granules freedomain,CGFD)形成,0.2mmol/L组也形成了CGFD,但1.0mmol/L和2.5mmol/L两个剂量组未观察到CGFD。1.0mmol/L和2.5mmol/LTGA可抑制p44/42MAPK的活化。结论:TGA可影响小鼠卵母细胞细胞质成熟和MAPK的活性,具有一定的生殖毒性。BACKGROUND AND AIM： To explore the effect of Thioglycolic acid （TGA） on cytoplasmic maturation and related molecular factors of mouse oocytes. MATERIALS AND METHODS：Mouse oocytes were matured in vitro cultured with serial doses of TGA. Immunoflurescence staining was used to label cortical granules （CGs） and p44/ 42MAPK was measured by westem blot. RESULTS： Rate of germinal vesicle-breakdown in each group reached 90% and comparison was insignificant （P 〉 0.05）. Rate of first polar body decreased with increasing TGA dose, the comparison between control and treatments was significant （P 〈 0.05）.CGs of oocytes from all groups migrated to the cortex and formed a continuous layer under the cell membrane, but CGs density in the cytoplasm became higher with increasing TGA treatment dose. Obvious cortical granule free domain （CGFD） was observed at 0 mmol/L TGA, in 0.2 mmol/L TGA CGFD could still be seen, but not in 1.0 mmol/L and 2.5 mmol/L TGA groups. Meanwhile, TGA inhibited p44/42MAPK activation in 1.0 mmol/L and 2.5 mmol/L treatment groups. CONCLUSION： TGA exerted reproductive toxicity since it interfered with cytoplasmic maturation and MAPK activation of mouse oocytes.

关 键 词：巯基乙酸 皮质颗粒 MAPK 卵母细胞 小鼠 

分 类 号：R730.45[医药卫生—肿瘤]